首页|短小芽孢杆菌DST27发酵培养基及发酵条件的优化

短小芽孢杆菌DST27发酵培养基及发酵条件的优化

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
[目的]通过优化生防菌株短小芽孢杆菌DST27发酵培养基及发酵培养条件,为后期该菌株的开发和应用提供基础.[方法]通过单因素筛选、Plackett-Burman试验和响应面分析法,以菌体生长量和对尖孢镰刀菌的抑菌活性为指标,优化DST27发酵培养基及发酵培养条件.[结果]最佳发酵培养基为1.35％葡萄糖、1％酵母粉和0.5％CaCl2;最佳发酵条件为装液量20％、接种量5.40％、初始pH 7.28、温度28 ℃和转速180r/min,在此培养条件下DST27的OD600为2.256,抑菌率为78.65％,与模型预测值81.42％相近,较优化前提高了 22.06％.[结论]该试验优化了 DST27发酵培养基及发酵条件,为今后该菌剂生产工艺及开展防治提供理论依据.
Optimization of fermentation medium and conditions for Bacillus pumilus DST27
[Aims]This study aims to establish a foundation for the later development and use of the biocontrol strain Bacillus pumilus DST27 by optimizing the fermentation medium and circumstances.[Methods]Based on single factor test,Plackett-Burman test and response surface analysis,the fermentation medium and conditions of DST27 were optimized with the bacterial biomass and the antifungal activity against Fusarium oxysporum as indicators.[Results]The optimal medium for liquid fermentation of strain DST27 was 1.35％glucose,1％yeast powder,and 0.5％CaCl2.The optimal fermentation conditions were as follows:liquid-loading rate 20％,inoculation amount 3％,pH 7.28,temperature 28 ℃,time 60 h,and rotating speed 180 r/min.The OD600 of DST27 was 2.256 under these cultivation circumstances,and the antifungal rate was 78.65％,which was similar to the predicted value of 81.42％in the model,and had increased by 22.06％compared to that before optimization.[Conclusions]This experiment optimized the fermentation medium and conditions of DST27,providing a theoretical basis for the production process of this bacterial agent and prevention with it in the future.

Bacillus pumilus DST27medium optimizationoptimization of fermentation conditionsPlackett-Burman designresponse surface analysis

李铮、王艳、晋玲、王伟、付瑶、栾涵钰、吕娇娇

展开 >

甘肃中医药大学药学院,兰州 730000

西北中藏药省部共建协同创新中心,兰州 730000

甘肃省中医药研究中心,兰州 730000

短小芽孢杆菌DST27 培养基优化 发酵条件优化 Plackett-Burman设计 响应面分析法

西北中藏药省部共建协同创新中心开放基金项目中药保障与创新能力提升项目甘肃省科技计划项目甘肃省2023年度优秀研究生"创新之星"甘肃省中医药研究中心开放课题

Xbzzy-2022-02甘中医药综函[2024]14号23ZDFA013-12023CXZX-763zyzx-2023-20

2024

10.16820/j.nyzz.2024.0705

农药
沈阳化工研究院有限公司

农药

CSTPCD北大核心
影响因子：0.665
ISSN：1006-0413
年,卷(期)：2024.63(7)
  • 17