Control efficacy of tebuconazole against twig blight of Phoebe by trunk injection and spraying methods
[Aims]This study was conducted to investigate the field control efficacy of tebuconazole against twig blight of Phoebe.[Methods]The biological activity of tebuconazole against the pathogen of twig blight of Phoebe,Lasiodiplodia pseudotheobromae,was determined through laboratory tests.Field plot trials for the control of twig blight of Phoebe were conducted using two application methods of trunk injection and spray.[Results]The EC50 of tebuconazole against L.pseudotheobromae was 1.7298 mg/L.After using tebuconazole 3%SL at 200 mL per plant via trunk injection,tebuconazole could be detected in various tissues of Phoebe on day 60,75,125,and 180 after application.The highest concentration was found on day 60,with mass fractions of 4.81,4.06 and 0.63 mg/kg in the xylem,phloem and leaves,respectively.In the field plot trials,the control efficacy against twig blight of Phoebe by trunk injection at 100 and 200 mL per plant were 76.19%and 83.33%,respectively.Spraying of tebuconazole at mass concentrations of 300 and 600 mg/L resulted in control efficacy of 78.57%and 88.10%,respectively.[Conclusions]Tebuconazole applied by trunk injection and spray method can effectively control twig blight of Phoebe,and can be applied in actual production.
twig blight of PhoebetebuconazoleLasiodiplodia pseudotheobromaetrunk injectionspray method