Determination of fenthion and its type Ⅰ metabolites in cowpea in Hainan by UPLC-MS/MS combined with QuEChERS
[Aims]A method was established for analysis of fenthion and its type Ⅰ metabolites in cowpea in Hainan by using QuEChERS purification combined with UPLC-MS/MS for determination.[Methods]The samples were extracted by acetonitrile,salted out with sodium chloride,purified with graphitized carbon black(GCB)and N-propylethylenediamine(PSA).The pesticides were separated with Agilent ZORBAX Eclipse Plus C18.Fenthion and its type Ⅰ metabolites were analyzed by UPLC-MS/MS with multiple reaction monitoring(MRM)mode.The solvent standard calibration curve was used for quantitative analysis with external standard method.[Results]The results showed that the good linear relationships were obtained for fenthion and its type Ⅰ metabolites in the concentration range from 2 to 100 pg/L.The correlation coefficients(r)were greater than 0.999.The average spiked recoveries for blank cowpea at 4 levels of 2,5,10,50 pg/kg were in the range of 89.3％to 99.3％,with relative standard deviations(RSD)in the range of 1.2％to 7.3％.The LOQs of the method were all 2.0 pg/kg.[Conclusions]The method is simple and cheap with high efficiency and accuracy,which is suitable to analyze fenthion and its type Ⅰmetabolites in cowpea in Hainan.This method will supply an effective technical support to supervise fenthion and its type Ⅰ metabolites in cowpea.
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