[Aims]This study aims to establish a method for the determination of fipronil and its metabolite residues in poultry and livestock meat by gas chromatography-electron capture detector.[Methods]The samples were extracted by acetonitrile and purified by n-hexane liquid-liquid distribution,the extracted solution was further purified with QuEChERS method to reduce matrix interference,the matrix effect was eliminated by matrix blank solution,and the components were separated by HP-5MS capillary column and analyzed by gas chromatography-electron capture detector.Quantitative analysis was carried out by external standard method.[Results]The fipronil and its three metabolite residues had good liner relationships in the range of 0.001 to 0.2 mg/L,with correlation coefficients more than 0.999.The recoveries of fipronil and its residues at 3 spiked levels(0.001,0.01,0.1 mg/kg)were 75.9％-119.4％,and the relative standard deviations were less than 5.0％.[Conclusions]The method is simple,convenient,sensitive and accurate,and is suitable for the analysis and detection of the residues of fipronil and its three metabolites in poultry and livestock meat.
关键词
家禽畜肉类/氟虫腈/代谢产物/GC-ECD
Key words
poultry and livestock meat/fipronil/metabolite/GC-ECD
维普
万方数据