Determination of residues of cyclohexenone herbicides and their metabolites in soil by liquid chromatography tandem mass spectrometry
[Aims]A method was established for the analysis and testing of 8 cyclohexenone herbicides and 2 metabolites in soil using ultrasonic assisted extraction and ultra-high performance liquid chromatography tandem mass spectrometry.[Methods]The soil samples were extracted by dichloromethane assisted with ultrasound,purified by aminated multi walled carbon nanomaterials,and separated by C18 chromatography column after solvent conversion,using 5 mmol/L ammonium formate aqueous solution and methanol as mobile phases for gradient elution,with multiple reaction monitoring(MRM)positive ion mode(ESI+)ionization scanning.Quantification was performed using a matrix matching standard curve external standard method.[Results]There was a good linear relationship between 8 cyclohexenone herbicides and 2 metabolites,with correlation coefficients greater than 0.999.The detection limit of the method was 0.01-0.1 μg/kg,with a quantification limit of 0.03-0.3 µg/kg.At the addition levels of high,medium and low blank samples,the average recovery rates of 8 cyclohexenone herbicides and 2 metabolites ranged from 71.81％ to 111.04％ ,with a relative standard deviation(RSD)of 0.46％ to 10.30％ (n=6).[Conclusions]This method is fast,accurate and easy to operate,which is suitable for residual detection of cyclohexenone herbicides and their metabolites in soil.
aminated multi walled carbon nanotubeliquid chromatography tandem mass spectrometrysoilcyclohexenoneherbicide
万方数据
维普
