Determination of residues of chlorantraniliprole and pymetrozine in rice by QuEChERS-UPLC-MS/MS
[Aims]This study aims to establish and optimize an analytical method based on ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)for simultaneous detection of chlorantraniliprole and pymetrozine in rice.[Methods]Brown rice samples were extracted with acetonitrile-acetic acid solution,while rice husk and straw samples were extracted with acetonitrile-aqueous ammonia solution.The extracts were purified by C18 and N-propyl ethylenediamine(PSA).ACQUITY UPLC® BEH C18 chromatographic column was used for separation and the mobile phase was methanol and 0.01％ formic acid-0.05％ ammonia aqueous solution.Electrospray ionization in positive ion mode was selected,the detection mode was multiple reaction monitoring(MRM),and external standard method was used for quantification.[Results]The average recoveries of chlorantraniliprole in brown rice,rice husk and straw were 91％ -102％ ,with relative standard deviations of 1.0％ -9.5％ .For pymetrozine,the average recoveries were 73％ -94％ ,with relative standard deviations of 1.1％ -5.8％ .The limits of quantitation for chlorantraniliprole and pymetrozine were both 0.01 mg/kg in brown rice,and 0.02 mg/kg in rice husk and straw.Both chlorantraniliprole and pymetrozine exhibited good linear relationships within the range of 0.001-0.5 mg/L,with correlation coefficients of 0.9996 and 0.9988,respectively.[Conclusions]This method is not only simple and rapid to operate but also exhibits high sensitivity,accuracy and precision,fully meeting the requirements for pesticide residue analysis in crops.Therefore,the analytical method established in this study can be widely applied to the residue analysis of chlorantraniliprole and pymetrozine in rice,providing a strong support for the quality and safety of agricultural products.
QuEChERSultra-high performance liquid chromatography-tandem mass spectrometryricechlorantraniliprolepymetrozine
万方数据
维普
