High performance liquid chromatography analysis of prohexadione calcium·(+)-abscisic acid 12％SC
[Aims]To improve the detection efficiency of prohexadione calcium·(+)-abscisic acid,a quantitative analytical method for the simultaneous detection of the active components of prohexadione calcium·(+)-abscisic acid 12％SC by high performance liquid chromatography(HPLC)was established.[Methods]The active components of the sample were separated and determined by a Hypersil GOLD C18 column under UV wavelength at 260 nm,with methanol and acetic acid water as the mobile phase.[Results]The results showed that the linear correlation coefficients of both prohexadione calcium and(+)-abscisic acid were 1.0000,and the standard deviations were 0.038 and 0.014,respectively.The coefficients of variation were 0.37％and 0.70％,and the average recovery rates were 106.43％and 98.47％,respectively.[Conclusions]The method applied here is simple to operate,effective in separation and cost-effective,and has high precision and accuracy.It is suitable for the quality control of prohexadione calcium·(+)-abscisic acid 12％SC.
prohexadione calcium(+)-abscisic acidHPLC analysisquality control
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