[Aims]To improve the detection efficiency of prohexadione calcium·(+)-abscisic acid,a quantitative analytical method for the simultaneous detection of the active components of prohexadione calcium·(+)-abscisic acid 12％SC by high performance liquid chromatography(HPLC)was established.[Methods]The active components of the sample were separated and determined by a Hypersil GOLD C18 column under UV wavelength at 260 nm,with methanol and acetic acid water as the mobile phase.[Results]The results showed that the linear correlation coefficients of both prohexadione calcium and(+)-abscisic acid were 1.0000,and the standard deviations were 0.038 and 0.014,respectively.The coefficients of variation were 0.37％and 0.70％,and the average recovery rates were 106.43％and 98.47％,respectively.[Conclusions]The method applied here is simple to operate,effective in separation and cost-effective,and has high precision and accuracy.It is suitable for the quality control of prohexadione calcium·(+)-abscisic acid 12％SC.
关键词
调环酸钙/S-诱抗素/高效液相色谱分析/质量控制
Key words
prohexadione calcium/(+)-abscisic acid/HPLC analysis/quality control
维普
万方数据