[Aims]The objective of this study is to establish and optimize a residue detection method for seboctylamine in potato by HPLC-MS/MS.[Methods]Potato tubers and plants were extracted by a mixture of 0.2％formic acid acetonitrile solution,soil samples were extracted with 0.2％ammonia acetonitrile solution.The extract was purified by combination of PSA,C18 and GCB,analyzed by HPLC-MS/MS,and quantified by external standard method.[Results]In the range of 0.005-0.5 mg/L,the linear relationship of seboctylamine in potato tubers,plants and soil was good.When the mass fraction was 0.02-0.5 mg/kg,the average recovery rate of seboctylamine in potato tubers,plants and soil was 77.42％-104.54％,the relative standard deviation(RSDs)were 2.70％-6.70％,and the limit of quantification(LOQ)was 0.02 mg/kg.The results showed that the half-lives of seboctylamine in potato plants and soil were 1.80-3.30 and 1.73-2.68 d,respectively,and the final residues of seboctylamine in potato tubers were lower than the limit of quantification.[Conclusions]This method is simple and rapid,which can provide data support for the formulation of the maximum residue level(MRL)of seboctylamine on potatoes,and provide a reference for its disease control and safety in potatoes.
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万方数据