Determination of tetraniliprole residue in fruits and vegetables by QuEChERS purification combined with HPLC-MS/MS
[Aims]The present study aims to establish a rapid analytical method for the determination of tetraniliprole residues in fruits and vegetables using QuEChERS purification combined with HPLC-MS/MS.[Methods]The samples were extracted with acetonitrile and purified using a QuEChERS purification package which consisted of anhydrous sodium sulfate(4 g),anhydrous magnesium sulfate(4 g),PSA(200 mg)and C18(100 mg).Separation was achieved on Agilent Venusil MP C18 column with gradient elution of methanol and water as the mobile phase.Detection was performed by electrospray ionization in negative ion scanning mode using multi-reaction monitoring(SRM)technique,while quantification was carried out through matrix matching external standard method.[Results]The correlation coefficients(r)of tetraniliprole within the linear range of 0.1-20 μg/L across various matrices consistently exceeded 0.999.The method exhibited a detection limit(S/N≥3)of 0.10 μg/kg and a limit of quantification(S/N≥10)of 0.50 μg/kg.Spiked recoveries at levels of 0.50,1.00 and 5.00 μg/kg in various matrices including Flammulina velutipe,carrot,leek,kidney bean,cabbage,tomato,cucumber,peach,rock candy orange,grape,pitaya and watermelon ranged from 82.86％to 99.15％with relative standard deviations(RSD,n=6)ranging from 0.10％to 6.79％.[Conclusions]The method is convenient,fast,and highly accurate and sensitive,fully meeting the requirements for detecting tetraniliprole residues in fruits and vegetables.
万方数据
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