Effects of different fertility promoting agents on the diversity of peripheral microbial population in Zizania latifolia
[Aims]Analyzing the changes in microbial population diversity in Zizania latifolia plants and soil after treated with different pesticides,in order to provide theoretical support for understanding the impact of pesticides on environmental microorganisms.[Methods]About 45 d after the field planting of Z.latifolia,different fertility promoting agents were used by leaf spray treatment.Four treatments were set,namely,water control(CK),fenaminosulf(H)and two plant endophytes(J,ZJ).Four weeks later,the samples of leaves and soil were collected,and the total DNA was extracted.With the high-throughput sequencing technology and bioinformatics methods,the effect of different treatments on the structure and diversity of bacterial and fungal communities was studied.[Results]The OTU/ASV analysis of bacterial and fungal communities showed that the bacterial OTUs and fungal OTUs on plants treated with fenaminosulf decreased by 33.03％and 14.18％respectively compared to the water control group,with little change in soil;that of the endophytes treatment group increased by 20.27％and 252.48％on plants,and increased by 5.08％and 25.64％in soil.The Alpha diversity analysis results showed that the Chao1 and Ace indices for measuring species richness were lower in the H group than in the CK group,while those in the J and ZJ groups were higher than in the CK group.The results of taxonomic analysis of species showed that the number of prokaryotic and eukaryotic organisms on the inter-root soil and plants of Z.latifolia treated with sodium trichlorfon reduced compared with that of the control of clear water at the level of phylum,class,order,family,genus and species.In contrast,prokaryotic and eukaryotic organisms did not reduce at the phylum,class,order,family,genus and species levels in the inter-root soil and plants of Z.latifolia after being treated with endophytes.[Conclusions]Fenaminosulf reduces the number and species richness of peripheral microbial communities in Z.latifolia,while endophyte enrich the composition of peripheral microbial communities,making microbial species more abundant.
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