Excessive use of organophosphorus(OP)and carbamate(CM)pesticides has seriously threatened human safety and health,and their detection remains limited by complex operations.A broad-spectrum and one-step rapid detection method for OP and CM pesti-cides was established by using digitally-controlled inkjet printing technology and cholinesterase inhibition method.Experimental results showed that test card has advantages of fast detection,good sensitivity,good stability,and high repeatability,which was suitable for real-time detection in various occasions,and has great application potential in POC detection.