Separation and Purification Process and Antioxidant Performance of Ficus carica L.Polysaccharides
To better utilize by-products of Ficus carica L.and improve their economic value,antioxidant properties of Ficus carica L.polysaccharides(FCW)were investigated.Microwave-assisted extraction of FCW was carried out,and extraction process was optim-ized.The optimal conditions were that,extraction temperature was 70 ℃,solid-liquid ratio was 1:40,microwave power was 800 W,and extraction time was 50 min.Under these conditions,extraction rate of FCW was 12.32％.The residue was used to simulate waste after Ficus carica L.production,Ficus carica L.residue polysaccharides(FCA)was extracted using alkaline extraction method,and analyze their thermal stability.Results showed that FCA had good thermal stability below 260 ℃.Polysaccharide component PFCA2 with a molecular weight of 28.188 kDa was obtained by separating and purifying it by ion exchange column and gel column.Results of monosaccharide composition showed that PFCA2 was mainly composed of rhammnose,glucose,arabinose,galactose,glucuronic acid,and galacturonic acid.In vitro antioxidant experiments showed that PFCA2 had good scavenging activities for DPPH free radicals and hy-droxyl free radicals,with IC50 values of 0.5 041 mg/mL and 3.2 802 mg/mL.This study showed that FCA have good antioxidant capa-city and were natural antioxidants.This study could provide a theoretical basis for deep processing of industrial by-products of Ficus carica L..
Ficus carica L.polysaccharideisolation and purificationphysical and chemical propertiesantioxidant activity
NETL
NSTL
万方数据
