破布叶组培快繁技术
Tissue culture and rapid propagation technology of Microcos paniculata L.
蔡清梅 1陈冬怡 2陈国华 2胡国弟 1周宏英3
作者信息
- 1. 广东慧达康制药有限公司,广东 佛山 528244
- 2. 中国科学院华南植物园,广东 广州 510650
- 3. 广东良田农林科技有限公司,广东 清远 511800
- 折叠
摘要
以破布叶茎段为外植体,通过植物组培快繁技术,培养成完整的脱毒植株.试验结果表明,外植体灭菌处理成功率 94%,诱导不定芽的最适培养基 1/4MS+0.5 mg/L 6-BA;不定芽继代增殖的最适培养基 1/4MS+0.5 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L GA3,增殖系数 3.3;最适出根培养基 1/4MS+1.0 mg/L IBA+0.1 mg/L NAA,出根率 93.1%;组培苗移栽成活率98%,为破布叶种苗提供了一种新型快速的繁殖方式,将促进岭南特色中药材破布叶高效新型产业化的形成和发展.
Abstract
Using stems of Microcos paniculata L.seedlings as explants,complete and virus-free plants were obtained by tissue culture and rapid propagation technology.Results showed that explants sterilization success rate was as high as 94%.Optimal medium for ad-ventitious buds induction was 1/4MS+0.5 mg/L 6-BA.Optimal medium for subculture multiplication was 1/4MS+0.5 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L GA3,and subculture multiplication factor was up to 3.3.Optimal medium for root was 1/4MS+1.0 mg/L IBA+0.1 mg/L NAA,while rooting rate was 93.1%.Transplanting survival rate was averaged at 98%.It could provide an effect way for rapid propagation of virus-free seedlings,which would promote formation and development of high efficiency and new industrialization of Microcos paniculata L.,a characteristic Lingnan medicinal plant.
关键词
破布叶/组培快繁/种苗/中药材Key words
Microcos paniculata L./tissue culture and rapid propagation/seed seedlings/Chinese medicinal herbs引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据