Tissue culture and rapid propagation technology of Microcos paniculata L.
Using stems of Microcos paniculata L.seedlings as explants,complete and virus-free plants were obtained by tissue culture and rapid propagation technology.Results showed that explants sterilization success rate was as high as 94%.Optimal medium for ad-ventitious buds induction was 1/4MS+0.5 mg/L 6-BA.Optimal medium for subculture multiplication was 1/4MS+0.5 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L GA3,and subculture multiplication factor was up to 3.3.Optimal medium for root was 1/4MS+1.0 mg/L IBA+0.1 mg/L NAA,while rooting rate was 93.1%.Transplanting survival rate was averaged at 98%.It could provide an effect way for rapid propagation of virus-free seedlings,which would promote formation and development of high efficiency and new industrialization of Microcos paniculata L.,a characteristic Lingnan medicinal plant.
Microcos paniculata L.tissue culture and rapid propagationseed seedlingsChinese medicinal herbs
万方数据
维普
