Effects of different straw return modes on bacterial diversity and community structure in tobacco-rice rotation soil
To explore the changes of soil bacterial diversity and community composition in tobacco-rice rotation soil under different straw return methods,control(CK),amendment(BP),undisturbed straw return(S0),straw-cow manure compost return(S1),straw-rapeseed cake compost return(S2),and these combined with amendment(S0BP,S1BP,S2BP)conditions were set up in a tobacco-rice rotation pot experiment.The composition of the soil bacterial community was analyzed using 16S rRNA high-throughput sequencing technology.The results of the experiment showed that direct straw return and straw return with amendment can effectively improve soil physical and chemical properties.Straw return combined with amendments significantly increased bacterial community abundance.The S1BP treatment resulted in the highest bacterial community abundance.Each treatment had no significant effect on the soil bacterial community diversity index.Proteobacteria(16.42%-26.01%),Chloroflexi(15.62%-20.46%),Acidobacteriota(8.71%-15.86%),Actinobacteriota(7.08%-11.47%)and Firmicutes(7.29%-11.74%)were the predominant phyla of soil bacterial communities.However,straw return significantly increased the relative abundances of Proteobacteria and Bacteroidota.Compared with straw return,the straw return with amendment treatments significantly increased the relative abundances of Bacteroidota and Desulfobacterota but markedly decreased the abundances of Acidobacteriota and Actinobacteriota.Furthermore,soil pH,minimum water holding capacity,soil porosity,available potassium,dissolved organic carbon,and microbial biomass carbon content were the main factors affecting bacterial community structure,which had a positive correlation with Desulfobacterota,Proteobacteria,and Bacteroidota but a negative correlation with Nitrospirota,Actinobacteriota,Acidobacteriota,and Armatimonadota.In summary,returning tobacco and rice straw directly or in combination with amendments can improve soil physicochemical properties and increase the abundance of soil bacterial communities.
万方数据
维普
