大蒜素微乳剂高效液相色谱分析方法研究
Research on the HPLC Analysis Method of Allicin Microemulsion
王玉萍 1付萍萍 1蒲时 1姜军侠1
作者信息
- 1. 陕西省农业检验检测中心,陕西 西安 710003
- 折叠
摘要
本文采用高效液相色谱法,以乙腈∶水=60∶40为流动相,使用C18色谱柱和二极管阵列检测器,在230 nm波长下对试样中的二烯丙基二硫醚、二烯丙基三硫醚进行分离和定量分析.结果表明二烯丙基二硫醚的线性相关系数为0.999 9,标准偏差为0.10%,变异系数为2.12%,平均回收率为100.79%;二烯丙基三硫醚的线性相关系数为0.996 1,标准偏差为0.03%,变异系数为2.19%,平均回收率为101.84%.
Abstract
diallyl disulfide and diallyl trisulfide in samples were separated and quantitatively analyzed by high performance liquid chromatography(HPLC)with acetonitrile and water(60∶40)as mobile phase,a C18 column and a diode array detector at a wavelength of 230 nm.The results showed that the linear correlation coefficient of diallyl disulfide was 0.999 9,with a standard devia-tion of 0.10%,a coefficient of variation of 2.12%,and an average recovery rate of 100.79%.Simi-larly,the linear correlation coefficient of diallyl trisulfide was 0.996 1,with a standard deviation of 0.03%,a coefficient of variation of 2.19%,and an average recovery rate of 101.84%.
关键词
二烯丙基二硫醚/二烯丙基三硫醚/高效液相色谱/分离/分析Key words
diallyl disulfide/diallyl trisulfide/HPLC/separation/analysis引用本文复制引用
基金项目
农药产品中有效成分测定通用分析方法液相色谱法体系建设项目()
出版年
2024
国家科技期刊平台
NSTL
万方数据