A method for separation and quantitative analysis of MCPA-EHE by HPLC with acetonitrile:water as mobile phase,Agilent Eclipse XDB-C18 column and at 230 nm wavelength was described.The result showed that the linear correlation coefficient of 0.999 8,the standard deviation of 0.13,the variation coefficient of 0.13%and the average recovery of 99.89%was obtained.