HPLC Method for Quantification of Picloram 150 g/L+Clopyralid 225 g/L Soluble Concentrate
A high performance liquid chromatography(HPLC)method was developed for the rapid analysis of Picloram 150 g/L+Clopyralid 225 g/L Soluble Concentrate mixed formulation.The separation and determination of Clopyralid and Picloram in the samples were performed on a CAPCELL PAK C18(250 mm×4.6 mm×5 μm)column with acetonitrile/methanol/phosphate water(pH 2.40)=20/10/70(V/V/V)as the mobile phase and by UV detector at 235 nm.The results showed that the linear correlation coefficient of Clopyralid and Picloram were 0.999 9 and 1.000 0,respectively,with coefficients of variation at 0.32% and 0.63% .The average recovery rates were 99.4% and 100.6%,meeting the requirements for quantitative control.The method developed in this paper is precise,accurate,simple and stable,and can be used for the quantitative analysis of Picloram and Clopyralid in single active ingredient preparations,mixed preparations,and technical materials under different specifications.
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