摘要
本文建立了高效液相色谱法检测茉莉花、百合、玫瑰花鲜样与干样中阿维菌素残留量的分析方法.样品采用乙腈提取,固相分散萃取净化,衍生后采用反相液相色谱荧光检测器检测,外标法定量.通过茉莉花、百合、玫瑰花空白样品分析表明,目标峰保留时间处无干扰,说明该方法特异性良好.阿维菌素峰面积与质量浓度在0.01～1.0 mg/L范围内具有良好的线性关系,R2为0.999 7.阿维菌素在鲜茉莉花、鲜玫瑰花、鲜百合中的平均添加回收率分别为92％～110％、84％～88％、86％～103％,相对标准偏差分别为2％～6％、6％～8％、5％～9％;在干茉莉花、干玫瑰花、干百合中的平均添加回收率分别为75％～87％、78％～89％、72％～88％,相对标准偏差分别为5％～12％、4％～8％、2％～12％;符合残留分析方法正确度和精密度的要求.定量限均为0.01 mg/kg.该方法具有良好的特异性、线性、定量限、正确度和精密度,适用于茉莉花、玫瑰花、百合中阿维菌素残留量的测定.
Abstract
This study establishes a method for the determination of abamectin residues in jasmine,lily and rose by high-performance liquid chromatography.The samples were extracted with acetonitrile,purified,and derivatized before analysis using reversed-phase high performance liquid chromatography(RP-HPLC)with FLD.The method demonstrated good specificity,as indicated by the absence of interference with the target peak in blank samples of jasmine,lily and rose.A good linear relationship between the peak area and the concentration of abamectin was observed over the range of 0.01～1.0 mg/L,with an R2 of 0.999 7.The average recoveries of abamectin,were 92％～110％in fresh jasmine,84％～88％in fresh rose,and 86％～103％in fresh lily.The relative standard deviations were 2％～6％for jasmine,6％～8％for rose and 5％～9％for lily.For dry flowers,the average recoveries were 75％～87％in jasmine,78％～89％in rose,and 72％～88％in lily,with RSD of 5％～12％,4％～8％and 2％～12％,respectively.The method meets the requirements of accuracy and precision for residue analysis,with a limit of quantification of 0.01 mg/kg.It offers good specificity,linearity,sensitivity,accuracy and precision,making it suitable for the determination of abamectin residue in jasmine,rose and lily.
维普
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