摘要
目的:通过网络药理学技术探究益气养阴化浊通络方治疗糖尿病肾病的分子机制.方法:通过 TCMSP 数据库获取益气养阴化浊通络方相关药物靶点,GeneCard、OMIM 及 PharmGkb 数据库获取糖尿病肾病相关靶点.Venny R软件获取益气养阴化浊通络方治疗糖尿病肾病的潜在靶点,Cytoscape 3.8.0 软件构建药物-有效成分-靶点-疾病网络图.对潜在靶点进行 PPI互作网络分析、GO 功能及 KEGG 通路富集分析.结果:共获取益气养阴化浊通络方有效成分 66 个,有效靶点 254 个,糖尿病肾病相关靶点 4755 个,取交集后获取潜在靶点 179 个.PPI 分析获取核心靶点 MAPK3、TP53、MAPK1、HSP90AA1、JUN、AKT1、RELA、MAPK14 等;GO 功能富集分析体现在对氧化应激的反应、细胞对化学压力的反应、对外来刺激的反应、对活性氧的反应等;细胞组分多在膜筏、膜微域、转录调节因子、复合囊泡腔等;分子功能多在 DNA结合转录因子结合、RNA 聚合酶 II特异性 DNA 结合转录因子结合、核受体活性、配体激活的转录因子活性等;KEGG 通路富集分析多在糖尿病并发症中的脂质与动脉粥样硬化、年龄-RAGE信号通路在糖尿病并发症中的作用、前列腺癌、乙肝、人巨细胞病毒感染、化学致癌-受体激活及流体切应力与动脉粥样硬化等通路.结论:通过网络药理学技术探究益气养阴化浊通络方治疗糖尿病肾病的作用机制,为后续相关研究提供理论依据和数据支撑.
Abstract
Objective:To explore the molecular mechanism of Yiqi Yangyin Huazhuo Tongluo decoction in the treatment of diabetic ne-phropathy through network pharmacology.Methods:Online database TCMSP was used to obtain Yiqi Yangyin Huazhuo Tongluo decoc-tion related drug targets,and online database GeneCard database,OMIM database and PharmGkb database were used to obtain diabetic nephropathy related targets.The two take the intersection to obtain the potential target of Yiqi Yangyin Huazhuo Tongluo decoction in the treatment of diabetic nephropathy.The potential targets were analyzed by PPI analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis.Results:A total of 66 active components in Yiqi Yangyin Huazhuo Tongluo decoction,254 effective targets and 4755 diabetic nephropathy related targets were obtained.179 potential targets were obtained after intersection.The core tar-gets MAPK3,TP53,MAPK1,HSP90AA1,JUN,AKT1,RELA,MAPK14 were obtained by PPI analysis,the functional enrichment anal-ysis of GO was reflected in the response to oxidative stress,chemical stress,external stimulation and reactive oxygen species,and the cellular components were mainly in membrane raft,membrane microdomain,transcriptional regulatory factors,complex vesicle cavity,etc.Most of the molecular functions are DNA binding transcription factor binding,RNA polymerase II specific DNA binding transcription factor binding,nuclear receptor activity,ligand activated transcription factor activity and so on.KEGG pathway enrichment analysis in-cluded lipid and atherosclerosis in diabetic complications,the role of age-rage signaling pathway in diabetic complications,prostate cancer,hepatitis B,human cytomegalovirus infection,chemical carcinogenic-receptor activation,fluid shear stress and atherosclerosis.Conclusion:To explore the mechanism of Yiqi Yangyin Huazhuo Tongluo decoction in the treatment of diabetic nephropathy through net-work pharmacology technology,so as to provide theoretical basis and data support for follow-up research.
基金项目
河南省中医药科学研究专项(2024ZYZD13)
河南省中医药科学研究专项(20-21ZY1069)
河南省科技攻关计划(23210231119)
南阳市科技攻关计划(KJGG104)
南阳理工学院大学生科研基金(NGDJ-2022-37)
NETL
NSTL
万方数据