Establishment of an Efficient and Stable Cabbage Transgenic System Mediated with Agrobacterium tumefactions and the Transformation of the CpTI gene
An efficient and stable cabbage transgenic system was established through the exploration of the factors affecting the Agrobacterium tumefactions transformation as well as the size and stage of explants.Six widely used cultivars were selected in this experiment.The hypocotyl segments from the sterile seedlings were used as the explants.Theexplants were infected with A.tumefaciens LBA4404 containing plasmidspBin-TI-19-2,in which constructed with two copies of the Cowpea Trypsin Inhibitor(CpTI)gene.The explants were screened on the medium containing kanamycin (50 mg/L).80% of the explants formed kanamycin-resistant callus,then differentiated into seedlings.One callus clump could forms 5 seedlings at minimum and 22 seedlings at maximum. The result of Southern hybridization with the kanamycin-resistant cabbages showed that the CpTI gene in the T-DNA had been fully integrated into the genome.The transgenic transformation efficiency reached to 20%.The insect-resistant test with Plutella xylostella（Linnaeus）demostrated that the resistance of the transgenic plants were stronger than the untransgenic plants.
国家科技期刊平台
NSTL
万方数据
维普
