Polymorphism of Osteopontin Gene (OPN) and Its Correlation with Egg-quality Traits in Laying Ducks (Anas platyrhyncha domestica)
Osteopontin (OPN) is a secreted phosphoprotein,playing important roles in eggshell formation.In order to analyze the correlation between the single nucleotide polymorphism (SNPs) of OPN and egg-quality traits of laying duck,the SNPs in the exon 7 of OPN gene were screened by PCR product sequencing in 100 Shanma laying duck and 100 Shaoxing laying duck (Anas platyrhyncha domestic).Eight SNPs in Shanma laying duck and 9 in Shaoxing laying duck were detected.The effects of G544C,A671G,G672C,T761C and T873C on some egg-quality traits of Shanma laying duck reached significant or highly significant difference levels.And the effects of A671G,G672C and T761C on some egg-quality traits of Shaoxing laying duck reached significant or highly significant difference levels.For the Shanma laying duck,3 genotypes in A671G,G672C,T761C,and T873C SNP sites were detected.Genotype BB of the sites A671G and G672C was significantly superior to the other two genotypes in 300-day-old's eggshell thickness (P＜0.01),while AA genotype was significantly higher in 300-day-old's Haugh unit (P＜0.05).BB genotype of the T761C site was significantly higher than the other two genotypes in 300-day-old's eggshell thickness (P＜0.01).BB genotype of the T873C site was significantly higher in 300-day-old's eggshell thickness and eggshell strength (P＜0.05)and 500-day-old's eggshell thickness,eggshell strength and eggshell weight (P＜0.05).Thus,we deduced that the allele B of T761C and allele B ofT873C were effective genes to egg-quality trait.For the Shaoxing laying duck,AA genotype of the T761C site was significantly lower than the other two genotypes in 300-day-old's eggshell thickness and eggshell strength (P＜0.05),while BB genotype was significantly lower than AA and AB in 500-day-old's egg shape index,egg weight,eggshell weight and weight of egg contents.The results indicate that OPN gene can be used as a molecular marker for egg-quality traits in MAS breeding of laying duck.
国家科技期刊平台
NSTL
万方数据
维普
