铁皮石斛PIN基因家族的全基因组鉴定和表达分析
Genome-wide Identification and Expression Analysis of PIN Gene Family in Dendrobium catenatum
王灏 1姜伟伟 1郭英纯 1斯金平 1陈东红1
作者信息
- 1. 浙江农林大学省部共建亚热带森林培育国家重点实验室,杭州311300;铁皮石斛产业国家创新联盟/国家林业和草原局铁皮石斛工程技术研究中心,杭州311300
- 折叠
摘要
生长素的极性运输和不对称分布主要由生长素极性输出载体PIN(PIN-FORMED)家族介导,对于植物生长发育具有重要的调控作用.本研究利用生物信息学和转录组测序等技术手段,对铁皮石斛(Dendrobium catenatum)PIN家族成员进行全基因组鉴定,并在系统进化关系、基因结构、蛋白保守基序和结构域组成、启动子顺式作用元件、组织表达谱和胁迫响应方面进行分析.结果显示,铁皮石斛有21个PIN家族成员,包括7个进化支(PIN1/2/3/5/6/8/9);在单子叶特有的PIN9进化支中,DcPIN9成员数量急剧扩增,可能与其特殊的生活方式和生存环境密切相关.DcPIN家族蛋白均含有标志性的N/C端疏水区(各含5个跨膜结构域)和中间的亲水环.DcPIN家族基因的上游启动子区域富含与激素、光、发育、逆境响应相关的顺式调控元件.转录组数据表明,DcPIN家族基因在不同组织中的表达存在明显差异,总体上可分为特异性表达和普遍性表达.不同处理下的转录组和qRT-PCR分析表明,DcPIN1a/9a/9d/9g参与对低温的响应,DcPIN9a/9f/9h参与对干旱的响应,DcPIN3a/9a/9d/9h参与对茉莉酸甲酯的响应,DcPIN1c/3a/9a参与对白绢病原翠雀小核菌(Sclerotium delphinii)侵染的响应.本研究为进一步解析铁皮石斛PIN基因家族在生长发育和环境适应中的功能提供参考依据.
Abstract
Auxin export carrier PIN-FORMED (PIN) family members mainly mediate auxin polar transport and asymmetric distribution. To better understand Dendrobium catenatum PIN (DcPIN) gene family, the present study performed genome-wide identification of DcPIN members, and further analyzed their phylogenetic relationships, gene structure, protein conserved motifs and domain composition, promoter cis-acting element, and expression profiles of both tissues and stress responses by means of bioinformatics and transcriptome data. The results showed that D. catenatum contained 21 DcPIN members that were divided into 7 clades. Among them, PIN9 clade was specific to monocot and contained high number of DcPIN9 genes in D. catenatum, which might be strongly relevant to its unique lifestyle and living environment. The upstream DcPIN promoters were rich in cis-regulatory elements related to hormones, light, development, and stress response. Transcriptome data showed the expression of DcPIN genes in different tissues could be totally divided into specific expression and constitutive expression. Transcriptome and qRT-PCR analysis under different treatments showed DcPIN1a/9a/9d/9g were in response to cold, DcPIN9a/9f/9h in response to drought, DcPIN3a/9a/9d/9h in response to JA, and DcPIN1c/3a/9a in response to the infection of Southern Blight pathogen Sclerotium delphinii. This result provides a theoretical basis for further investigating the function of D. catenatum PIN genes in the growth and development and environmental adaptation.
关键词
铁皮石斛/PIN基因家族/生长素/极性运输/基因表达Key words
Dendrobium catenatum/PIN gene family/Auxin/Polar transport/Gene expression引用本文复制引用
基金项目
出版年
2021
国家科技期刊平台
NSTL
维普
万方数据