马铃薯休眠解除相关基因StNF-YA8的表达分析
Expression Analysis of the StNF-YA8 Gene Associated with Dormancy Release in Potato(Solanum tuberosum)
王霄 1魏涵 2李世贵 2王凯彤 1赵亮 3张宁 1司怀军1
作者信息
- 1. 甘肃农业大学生命科学技术学院,兰州 730070;甘肃农业大学省部共建干旱生境作物学国家重点实验室,兰州 730070
- 2. 甘肃农业大学生命科学技术学院,兰州 730070
- 3. 新疆农业科学院奇台麦类试验站,奇台 831800
- 折叠
摘要
核因子Y(nuclear factor Y,NF-Y)是真核生物中分布较为广泛的一类转录因子,由NF-YA、NF-YB和NF-YC三种亚基构成,NF-YA参与调控植物的生长发育及胁迫响应.本研究用qRT-PCR方法分析了10个马铃薯(Solanum tuberosum)StNF-YA基因在块茎休眠解除过程中的表达模式.结果表明,StNF-YA8基因在块茎休眠解除过程中持续上调表达.进一步克隆了StNF-YA8基因(GenBank No.XM_015309982.1),其ORF为591 bp,编码196个氨基酸.构建了StNF-YA8蛋白的亚细胞定位载体,结果显示,StNF-YA8蛋白定位在细胞核和细胞膜上.组织特异性分析发现,StNF-YA8基因在不同组织间存在表达差异.生物信息学分析表明,马铃薯StNF-YA8蛋白含有CBFB保守结构域,在进化程度上与野生马铃薯(S.commersonii)亲缘性最高;StNF-YA8蛋白与StNF-YB3/5/10/14/17和StNF-YC1/4/5/8/9蛋白互作;该基因启动子序列中存在参与光响应、昼夜节律控制和激素响应相关的顺式作用元件.本研究为深入解析马铃薯StNF-YA8基因参与调节块茎休眠解除的分子机制提供了基础资料.
Abstract
Nuclear factor-Y(NF-Y)is a class of transcription factors widely distributed in eukaryotes.It composed of NF-YA,NF-YB and NF-YC subunits.NF-YA is involved in regulating plant growth and development,and stress response.In this study,qRT-PCR method was used to analyze the expression pattern of 10 StNF-YA genes of potato(Solanum tuberosum)in the process of tuber dormancy release.The results showed that the StNF-YA8 gene was consistently up-regulated in expression during dormancy release in tubers.The StNF-YA8 gene was further cloned(GenBank No.XM_015309982.1),and its ORF was 591 bp,encoding 196 amino acids.The subcellular localization vector of StNF-YA8 protein was constructed,and the results showed that StNF-YA8 protein was localized in the nucleus and cell membrane.Tissue-specific analysis revealed that the StNF-YA8 gene was differentially expressed between different tissues.The results of bioinformatics analysis showed that the potato StNF-YA8 protein contained a conserved CBFB domain,and had the highest evolutionary relationship with wild potato(S.commersonii);StNF-YA8 protein closely interacted with StNF-YB3/5/10/14/17 and StNF-YC1/4/5/8/9 proteins;there were cis-acting elements involved in light response,circadian rhythm control and hormone response in the promoter sequence of this gene.The results can provide a basis for the molecular mechanism analysis of potato StNF-YA8 gene involved in regulating tuber dormancy release.
关键词
马铃薯/休眠/表达分析/核因子YA8(StNF-YA8)/生物信息学分析Key words
Potato/Dormancy/Expression analysis/Nuclear factor YA8(StNF-YA8)/Bioinformatics analysis引用本文复制引用
基金项目
国家自然科学基金(31960444)
甘肃省科技重大专项(GZGG-2021-6)
科技部对发展中国家科技援助项目(KY201901015)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据