过表达F-box基因BoFBX117对甘蓝低温耐受性的影响
Effect of Overexpression of F-box Gene BoFBX117 on Low-temperature Tolerance of Cabbage(Brassica oleracea var.capitata)
罗玉霞 1许涛 1戴倩 1陈瑶 1宋江华1
作者信息
- 1. 安徽农业大学园艺学院,合肥 230036
- 折叠
摘要
F-box基因在植物非生物胁迫响应过程中发挥重要作用.为了研究F-box基因BoFBX117(GenBank No.XM_013731217.1)在甘蓝(Brassica oleracea var.capitata)响应低温胁迫中的功能,本研究构建了BoFBX117过表达载体,通过农杆菌介导法获得过表达BoFBX117甘蓝(OE-BoFBX117)植株.在4、0、-2和-4℃低温胁迫24h后,通过观察表型发现过表达BoFBX117甘蓝叶片黄化萎蔫程度均小于非转基因甘蓝(WT);经过7d恢复期,发现过表达BoFBX117甘蓝恢复正常生长能力更强.qPCR结果表明,低温胁迫下转基因植株中BoFBX117的表达量显著升高(P＜0.01),是WT植株的1.87～4.80倍.0℃低温胁迫时,转基因植株的超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和过氧化氢酶(Catalase,CAT)活性最高,分别比WT植株提高 80%、16%和 99%.低温胁迫下,丙二醛(malondialdehyde,MDA)含量不断积累,-4℃时转基因植株的丙二醛含量为0.62 μmol/g FW,比WT降低53.38%.在正常条件和低温胁迫下,转基因植株的脯氨酸(proline)和可溶性糖(soluble sugar)含量均显著高于WT植株(P＜0.05).结果表明,BoFBX117 在低温胁迫应答过程中发挥正调控作用,过表达BoFBX117提高了转基因甘蓝的低温耐受性,本研究为甘蓝耐低温品种选育提供支持.
Abstract
F-box gene plays an important role in plant abiotic stress response.In order to study the function of F-box gene BoFBX117(GenBank No.XM_013731217.1)in cabbage(Brassica oleracea var.capitata)response to low-temperature stress,in this study,the BoFBX117 over-expression vector was constructed,and the over-expression of BoFBX117 cabbage(OE-BoFBX117)plants were obtained by Agrobacterium tumefaciens mediated method.After 24 h of low-temperature stress at 4,0,-2 and-4℃,the phenotype of the over-expression of BoFBX117 cabbage showed that the degree of leaf yellowing wilting was less than that of non-transgenic cabbage(WT).After 7 d of recovery,it was found that the over-expression of BoFBX117 cabbage had stronger ability to return to normal growth.The results of qPCR showed that the expression of BoFBX117 in transgenic plants was significantly increased under low-temperature stress(P＜0.01),which was 1.87～4.80 times that of WT plants.Under low-temperature stress at 0℃,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)of transgenic plants were the highest,which were 80%,16%and 99%higher than those of WT plants respectively.Under low-temperature stress,the content of malondialdehyde(MDA)continuously accumulated.At-4℃,the MDA content of transgenic plants was 0.62 μmol/g FW,53.38%lower than WT.Under normal conditions and low-temperature stress,the proline and soluble sugar contents of transgenic plants were significantly higher than those of WT plants(P＜0.05).The results showed that BoFBX117 might play a positive role in the response to low-temperature stress,and over-expression of BoFBX117 in cabbage could improve the low-temperature tolerance of cabbage.This study provides support for the breeding of low-temperature tolerance varieties of cabbage.
关键词
甘蓝/F-box基因/BoFBX117/低温胁迫Key words
Brassica oleracea var.capitata/F-box gene/BoFBX117/Low-temperature stress引用本文复制引用
基金项目
国家自然科学基金(32272728)
国家自然科学基金(31872108)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据