摘要
性别是影响绵羊(Ovis aries)骨骼肌发育和产肉性能的主要因素之一.本研究以不同性别8月龄绵羊为研究对象,利用转录组测序技术探究不同性别绵羊背最长肌中基因表达差异,旨在揭示性别对绵羊骨骼肌发育影响的分子机理.经转录组测序和分析,共鉴定到14 587个基因,其中显著差异表达基因共29个,公羊组比母羊组显著上调表达23个,下调表达6个,差异基因包括xin肌动蛋白结合重复内含物1(xin actin binding repeat containing 1,XIRP1)、谷胱甘肽过氧化物酶2(glutathione peroxidase 2,GPX2)、酰基辅酶A合成酶中链家族成员1(acyl-CoA synthetase medium chain family member 1,ACSM1)、脂肪酸结合蛋白4(fatty acid binding protein 4,FABP4)、睾丸特异性丝氨酸激酶6(testis specific serine kinase 6,TSSK6)等.对各组间差异表达基因进行GO和KEGG分析,GO富集分析显示,差异表达基因主要富集在核糖核苷、肌动蛋白结合和细胞骨架结合等类别中.KEGG通路分析发现,差异表达基因显著富集到3个信号通路中,分别为PPAR信号通路、花生四烯酸代谢和谷胱甘肽代谢信号通路.因此,不同性别间绵羊骨骼肌发育的差异可能通过以上通路产生.随机选取8个差异表达基因,利用qRT-PCR对其表达量进行验证,结果与转录组测序结果基本一致,证明测序结果可靠.综上,本研究揭示了不同性别绵羊骨骼肌基因表达的差异及相关的信号通路,为进一步了解绵羊性别影响肌肉发育的机制提供了参考.
Abstract
Sex is one of the main factors affecting skeletal muscle development and meat production performance in sheep(Ovis aries).This study investigated the differences in gene expression in sheep longest dorsal muscle of different sexes using transcriptome sequencing technology,aiming to reveal the molecular mechanism of the effect of sex on skeletal muscle development in sheep.A total of 14 587 genes were identified by transcriptome sequencing and analysis,among which 29 genes were significantly differentially expressed.23 genes were significantly up-regulated and 6 were down-regulated in the ram group compared with the ewe group.The differential genes included xin actin binding repeat containing 1(XIRP1),glutathione peroxidase 2(GPX2),acyl-CoA synthetase medium chain family member 1(ACSM1),fatty acid binding protein 4(FABP4),testis specific serine kinase 6(TSSK6),etc.The GO analysis showed that the differentially expressed genes were mainly enriched in ribonucleoside,actin-binding and cytoskeleton-binding categories,and the KEGG pathway analysis revealed that the differentially expressed genes were significantly enriched in 3 signaling pathways,namely PPAR signaling pathway,arachidonic acid metabolism and glutathione metabolism signaling pathway.Thus,differences in skeletal muscle development in sheep between the sexes may arise through the above pathways.Eight differentially expressed genes were randomly selected and their expression was verified by qPCR,and the results were generally consistent with the transcriptome sequencing results,which proved that the sequencing results were reliable.In conclusion,this study revealed the differences in skeletal muscle gene expression and related signaling pathways in sheep of different sexes,which provides reference for further understanding the mechanisms by which sex affects muscle development in sheep.
基金项目
国家肉羊产业技术体系(CAS38)
安徽省肉羊良种联合攻关(2021)()
安徽省自然科学基金优秀青年基金(2108085Y11)
国家科技期刊平台
NSTL
维普
万方数据