摘要
急性软组织损伤和烫伤是兽医临床上常见的外伤疾病,均以局部急性无菌性炎症反应和组织损伤修复为主要的病理变化特点.目前,在兽医领域,对促进组织损伤修复的药物研究较少,复方黄龙软膏是依据中兽医经验方研制而成的促组织损伤修复的中药软膏,具有较好的临床疗效.本研究对复方黄龙软膏的药效进行初步探索,建立了重物击打SD大鼠(Rattus norvegicus)急性软组织损伤模型和热金属高温烫伤C57BL/6J小鼠(Mus musculus)模型,通过症候表现变化和苏木精-伊红(haematoxylin and eosin,HE)染色病理组织切片观察,评估复方黄龙软膏促急性软组织损伤及烫伤修复的药效.结果表明,促急性软组织损伤修复试验中,用药72h时,复方黄龙软膏组与模型组相比,急性软组织损伤大鼠症候评分极显著下降(P＜0.01),后肢肿胀率极显著降低(P＜0.01);复方黄龙软膏组与阳性药物(双氯芬酸二乙胺乳胶)组相比,肿胀率显著降低(P＜0.05).促烫伤修复试验中,用药7d时,复方黄龙软膏组小鼠烫伤病灶愈合率为(47.82±5.53)%,是模型组愈合率的1.4倍,差异显著(P＜0.05).病理切片结果表明,与模型组相比,复方黄龙软膏组中大鼠软组织和小鼠皮肤中的出血和炎症征象均减轻,胶原纤维增多.本研究证明,复方黄龙软膏能显著改善急性软组织损伤模型大鼠的瘀斑、水肿等症状,抑制炎症反应,促进损伤组织修复,且与双氯芬酸二乙胺乳膏相比,在消除水肿方面更具优势.在促烫伤修复方面,能加快烫伤模型小鼠烫伤创面愈合,促进其创面修复,并抑制炎症反应.本研究为复方黄龙软膏的临床应用提供初步理论依据.
Abstract
Acute soft tissue injury and scald are common types of traumatic diseases in veterinary clinics,both characterized by local acute aseptic inflammatory response and tissue damage repair as the main pathological changes.In the field of veterinary medicine,there is not much research on drugs that promote tissue damage repair at present.Compound Huang-Long Cream is a Chinese herbal ointment for promoting tissue damage repair according to the empirical formula from Traditional Chinese Veterinary Medicine,which has a good clinical efficacy.This study aimed to conduct a preliminary exploration of the therapeutic effect of Compound Huang-Long Cream and provide a experimental basis for its development and clinical application.An acute soft tissue injury model in SD rats(Rattus norvegicus)and a scald model in C57BL/6J mice(Mus musculus)with high-temperature mental scald were established.The changes of symptomatic manifestations and the haematoxylin and eosin(HE)staining were used to evaluate the pharmacodynamics efficacy of Compound Huang-Long Cream in promoting the repair of acute soft tissue injury and scald injury.The results showed that in the acute soft tissue injury repair experiment,the syndrome score of rats with acute soft tissue injury decreased extremely significantly(P＜0.01)and the swelling rate of hind limbs decreased extremely significantly(P＜0.01)in the Compound Huang-Long Cream group compared with the model group at 72 h after molding.There was a significant difference(P＜0.05)in the swelling rates in the Compound Huang-Long Cream group compared with the positive drug(Diclofenac Diethylamine Emulgel)group.In the scald injury repair experiment,the healing rate of scald focus in the Compound Huang-Long Cream group was(47.82±5.53)%,which was 1.4 times higher than that in the model group(P＜0.05)on the day 7.The pathological section results showed that compared with the model group,hemorrhage and inflammation were reduced and collagen fibers were increased in both the soft tissue of rats and the skin of mice in the Compound Huang-Long Cream group.This study indicates that Compound Huang-Long Cream can significantly improve the symptoms of ecchymosis and edema,inhibit inflammation and promote the repair of damaged tissues in rats with acute soft tissue injury model.Compared with diclofenac diethylamine emulgel,it was better at eliminating edema.In terms of promoting scald repair,Compound Huang-Long Cream could accelerate the healing of scald wounds in scald model mice,promoted their wound repair,and inhibited inflammation.This study provides a preliminary experimental basis for the clinical application of Compound Huang-Long Cream.
基金项目
十四五国家重点研发计划课题(2022YFD1801104)
中国农业大学2115人才工程(00109023)
国家科技期刊平台
NSTL
维普
万方数据