陆地棉磷脂酶C基因家族的鉴定及其在棉纤维发育过程中的表达分析
Identification of Gossypium hirsutum PLC Gene Family and Its Expression Analysis During Fiber Developmental Stages
窦玲玲 1牛换换 1郑凯2
作者信息
- 1. 咸阳师范学院化学与化工学院,咸阳 712000
- 2. 新疆农业大学农学院,乌鲁木齐 830000
- 折叠
摘要
磷脂酶C(phospholipase C,PLC)是参与磷脂酰肌醇4,5-二磷酸水解的酶,其下游产物介导Ca2+和蛋白激酶C的信号转导.本研究利用磷脂酶C的隐马尔可夫模型(PF09279和PF04185)从陆地棉(Gossypium hirsutum)'农大棉8号'(NDM8)基因组中鉴定到28个GhPLC基因家族成员.蛋白质序列系统发育分析将GhPLC家族成员分为PIPLC(16个)和nPLC(12个)两大亚组;基因复制分析显示,50%的GhPIPLC和92%的GhnPLC基因起源于片段重复,且受到纯化选择;GhPLC家族基因上游2000 bp序列启动子顺式作用元件分析显示,所有的GhPLC基因启动子均含有应答植物激素或非生物胁迫的顺式作用元件,表明其可能被植物激素或非生物胁迫诱导表达;qPCR结果显示,15个含有响应赤霉素的顺式作用元件的GhPLC基因中的14个可以被赤霉素诱导上调表达;含有响应生长素的顺式作用元件的15个GhPLC基因中的14个可以被生长素类似物诱导上调表达;25个GhPLC家族基因在棉纤维起始和伸长阶段上调表达,占GhPLC基因家族成员的89%.本研究为后续深入研究GhPLC基因在棉纤维起始和伸长过程中的功能提供了参考.
Abstract
Phospholipase C(PLC)is an enzyme involved in the hydrolysis of phosphatidylinositol 4,5-diphosphate,and its downstream products are involved in the signal transduction of Ca2+ and protein kinase C.In this study,28 members of the GhPLC gene family were identified from the genome of Gossypium hirsutum'Nongdamian No.8'(NDM8)by using the Hidden Markov Model(PF09279 and PF04185).According to the phylogenetic analysis of protein sequences,GhPLC family members were divided into 2 subgroups:PIPLC(16)and nPLC(12).Gene duplication analysis showed that 50%of GhPIPLC and 92%of GhnPLC genes originated from segmental duplication and subjected to purification selection.The cis-acting elements analysis of the upstream 2000 bp sequence of GhPLC family genes as promoters found that all GhPLC gene promoters contained plant hormone or abiotic stress responsive cis-acting elements,indicating that GhPLCs could be induced by plant hormone or abiotic stresses.qPCR analysis showed that 15 GhPLC gene promoters contained gibberellin responsive-element,and 14 of them could be induced by gibberellin to up-regulate the expression.15 GhPLC gene promoters contained auxin-responsive elements,among which 14 GhPLC could be induced by auxin analogues to up-regulate the expression.25 of the 28 GhPLC gene family members were up-regulated in the initiation and elongation stages of cotton fiber developmental stages,accounting for 89%of the GhPLC gene family members.This study provides a reference for further study on the function of GhPLC gene in cotton fiber initiation and elongation stages.
关键词
陆地棉/PLC基因家族/进化分析/启动子/表达分析Key words
Gossypum hirsutum/PLC gene family/Evolutionary analysis/Promoter/Expression analysis引用本文复制引用
基金项目
陕西省教育厅专项科研项目(22JK0602)
陕西省教育厅专项科研项目(20JK0973)
陕西省大学生创新创业训练计划(S202310722092)
安徽荃银高科种业股份有限公司项目(GMLM2023-09)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据