茭白'浙农7号'不同生长阶段的叶片转录组学及激素水平分析
Analysis of Transcriptomics and Hormone Levels of Jiaobai'zhenong 7'in Different Growth Stages
张珏锋 1李芳 1钟海英 1陈建明1
作者信息
- 1. 浙江省农业科学院植物保护与微生物研究所,杭州 310021
- 折叠
摘要
茭白是由菰黑粉菌(Ustilago esculenta)侵染刺激菰(Zizania latifolia)植株,使其内源激素发生变化,促使寄主进入孕茭阶段并刺激植株茎秆部膨大而形成.明确茭白不同生长阶段生育期激素以及转录组水平差异,对发现与茭白生育期调控相关的功能基因有重要意义.本研究采用Illumina平台测序技术对茭白新品种'浙农7号'('zhenong 7',ZN7)分蘖前期、分蘖末期、孕茭前期、孕茭末期4个生长阶段进行转录组测序分析.结果表明,4个生长阶段共筛选出差异表达基因(differentially expressed genes,DEGs)194个;4个生长阶段排名前30的单一基因GO(Gene Ontology)分类结果显示,在level 2水平上,以归类于植物对光反应的GO条目在各组比对中居多,包括细胞对强光的反应(cellular response to high light intensity)以及细胞对紫外线-A(ultraviolet-A,UV-A)的反应(cellular response to UV-A)等;KEGG富集分析结果表明,差异表达基因主要参与光合作用及光合作用-触角蛋白及光合生物中的碳固定等.比较4个生长阶段内源激素含量发现,赤霉素(gibberellin,GA3)、生长素(auxin,IAA)、脱落酸(abscisic acid,ABA)和细胞分裂素(cytokinin,CTK)在茭白生长各阶段的含量变化趋势并不一致.赤霉素在孕茭期的含量显著高于分蘖期(P＜0.05);生长素与脱落酸含量在各个时期变化趋势基本一致,均为分蘖末期＞分蘖前期＞孕茭期;细胞分裂素的含量在分蘖前期达到顶峰,显著高于分蘖末期及孕茭期(P＜0.05),而其余3个阶段之间并无显著差异.研究结果为阐明茭白不同生长阶段关键基因及其调控机理提供科学依据.
Abstract
Jiaobai is formed when Ustilago esculenta infects and stimulates the Zizania latifolia plant,causing endogenous hormones changes that induce the host pregnancy and stimulate the stem of the plant to swell.So determination of dynamic changes of hormones and transcriptome is important to clarify the regulation mechanism of key genes in different stages of growth and development of Jiaobai.Illumina platform sequencing technology was used to sequence the transcripts of 4 growth stages(pre tillering stage,tillering stage,pre pregnancy stage and pregnancy stage)of Jiaobai varieties'zhenong 7'(ZN7)in this study.The results showed that total of 194 differentially expressed genes(DEGs)were screened in 4 growth stages,Gene Ontology(GO)function analysis showed that the DEGs in 4 growth stages of ZN7 were mainly involved in cell response to high light intensity and cell response to ultraviolet-A(UV-A)biological process.DEGs were mainly involved in photosynthesis,photosynthesis-antennal protein,carbon fixation in photosynthetic organisms etc.in KEGG enrichment analysis.Compared the contents of endogenous hormones in the 4 growth stages of ZN7,it was found that the trend of content changes of gibberellin(GA3),cytokinin(CTK),auxin(IAA)and abscisic acid(ABA)in each growth stage of Jiaobai were not consistent.The content of GA3 in pregnant period was significantly higher than that in tillering stage(P＜0.05),contents of IAA and ABA in each period were basically the same,which was late tillering stage＞early tillering stage＞pregnant stage,the CTK content reached the peak in the early tillering stage,which was significantly higher than that in the late tillering stage and pregnant stage(P＜0.05),but there was no significant difference among the other 3 stages.The results provide scientific basis for key genes screening and regulatory mechanisms illustrating at different growth stages of Jiaobai.
关键词
茭白'浙农7号'/不同生长阶段/转录组/差异表达基因(DEGs)/内源激素Key words
Jiaobai'zhenong 7'/Different growth stages/Transcriptome/Differentially expressed genes(DEGs)/Endogenous hormone引用本文复制引用
基金项目
现代农业产业技术体系建设项目(CARS-24-G-07)
浙江省"三农九方"科技协作计划(2022SNJF016)
浙江省"三农九方"科技协作计划(2022SNJF082)
国家自然科学基金青年基金(32001963)
出版年
2024
国家科技期刊平台
NSTL
万方数据