摘要
4-香豆酸辅酶A连接酶(4-coumarate coenzyme A ligase,4CL)是苯丙烷代谢途径的关键性限速酶,可以调控黄酮、花青素和木质素的合成,对植物应对不良环境胁迫具有重要作用.为研究掌叶覆盆子(Rubus chingii)4CL基因家族的生物学功能,本研究通过生物信息学的方法,在掌叶覆盆子基因组中鉴定得到4CL家族基因,对各成员进行理化性质和亚细胞定位预测、染色体定位、系统进化、基因结构和保守基序、蛋白二级结构以及顺式作用元件等分析,并检测其在掌叶覆盆子不同组织器官和不同生长时期的表达水平,以揭示掌叶覆盆子中4CL基因家族的成员特性和表达模式.结果表明,掌叶覆盆子4CL基因家族有6个成员,理化性质较为相似,基因结构保守,被分成3个亚家族,不均匀地分布于2、3、4、5和7号染色体上.Rc4CL基因启动子区域存在多个顺式作用元件,响应生长素、赤霉素、茉莉酸、脱落酸、光照、厌氧、干旱等多种激素或胁迫诱导.Rc4CL家族基因在不同组织器官中的表达存在显著差异,Rc4CL1、Rc4CL2、Rc4CL3、Rc4CL4在根中高丰度表达,Rc4CL5和Rc4CL6在果实中高丰度表达.在果实不同发育阶段的表达也存在显著差异,Rc4CL1、Rc4CL2和Rc4CL3的表达水平在果实发育后期呈下降趋势;Rc4CL2、Rc4CL5和Rc4CL6在果实发育后期表达水平较高,表明其在果实成熟中起着重要的作用.本研究为深入研究掌叶覆盆子Rc4CL基因及其生物学功能提供参考.
Abstract
4-coumarate coenzyme A ligase(4CL)is the last key enzyme of the phenylpropane pathway,which can regulate the biosynthesis of lignin,anthocyanin,and flavonoids,and has an important effect on stress resistance.In order to reveal the biological role of 4CL gene family in Rubus chingii,the present study identified the 4CL gene family through an approach of bioinformatics,and investigated the physicochemical properties,subcellular localization,chromosomal localization,systematic evolution,gene structure,conserved motifs,protein secondary structure and cis-acting elements of each member.Besides,their expression profiles in different tissues and at different growth stages were detected.The results showed that 64CL gene family members were presented in R.chingii,with similar physicochemical properties and conserved gene structure,and were divided into 3 subfamilies.Six 4CL genes were unevenly distributed on chromosomes 2,3,4,5 and 7.The promoter regions of 64CL genes harbored auxin,gibberellin,jasmonic acid,abscisic acid,light,anaerobic,and drought responsive cis-acting elements.The 4CL genes differentially expressed in different R.chingii tissues,among them,Rc4CL1,Rc4CL2,Rc4CL3 and Rc4CL4 were highly expressed in roots,while Rc4CL5 and Rc4CL6 were highly expressed in fruits.Simultaneously,they also differentially expressed at different fruit growth stages,among them,Rc4CL1,Rc4CL2 and Rc4CL3 showed a downward trend at the late stage of fruit development.However,Rc4CL2,Rc4CL5 and Rc4CL6 were highly expressed at the late stage of fruit development,indicating that they might play a crucial role in fruit ripening.The results provide a reference for in-depth study of the 4CL gene and its biological role in R.chingii.
基金项目
国家自然科学基金(32000257)
浙江省农业新品种培育重大科技项目(2021C02074)
浙江省基础公益研究计划(LY23H280003)
浙江中医药大学科研项目(2022JKZKTS15)
国家科技期刊平台
NSTL
维普
万方数据