运输应激对山羊胃壁组织细胞凋亡及凋亡相关基因Bcl-2和Bax表达的影响
Effects of Transport Stress on Apoptosis and Expression of Apoptosis-related Genes Bcl-2 and Bax in the Gastric Wall Tissues of Goat(Capra hircus)
胡威 1刘犇 2郑文亚 2方满新 3胡迎东 3章志涛 3张雯苑 3叶恬3
作者信息
- 1. 宜春学院生命科学与资源环境学院,宜春 336000;江西绿科农牧科技有限公司,宜春 336000
- 2. 宜春学院生命科学与资源环境学院,宜春 336000;江西省高等学校硒农业工程技术研究中心,宜春 336000;江西绿科农牧科技有限公司,宜春 336000
- 3. 宜春学院生命科学与资源环境学院,宜春 336000
- 折叠
摘要
随着山羊(Capra hircus)养殖业的发展,交通运输引起的山羊健康问题已不容忽视.本研究旨在探讨运输应激对赣西山羊胃壁组织细胞凋亡及凋亡相关基因B细胞淋巴瘤-2(B-cell lymphoma-2,Bcl-2)和Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)表达的影响.将12只1周岁的健康赣西公山羊随机均分为:对照组(n=4)、2 h运输组(n=4)、6 h运输组(n=4).运输过程中禁食、禁水,待运输结束后收集山羊各胃壁的组织并进行相关的组织学和定量分析.末端脱氧核苷酸转移酶介导的dUTP生物素缺口末端标记法(terminal dexynucleotidyl transferase-mediated dUTP-biotin nick end labeling,TUNEL)结果显示,与对照组相比,2 h运输组和6h运输组山羊胃壁组织的细胞凋亡率发生了显著升高(P＜0.05),并且除瓣胃外其余胃壁组织6h运输组细胞凋亡率显著高于2h运输组(P＜0.05);免疫组织化学结果显示,Bax和Bcl-2主要定位于前胃(瘤胃,网胃,瓣胃)的黏膜上皮和皱胃的固有层,与对照组相比,两运输组中Bax在各胃壁上述部位的表达增强,而Bcl-2的表达减弱(除瓣胃外);qPCR结果显示,两运输组各胃壁组织中Bax的mRNA表达水平除瓣胃外均发生了显著上调(P＜0.05),而Bcl-2的mRNA表达水平均发生了显著下调(P＜0.05),Bax/Bcl-2的比率发生了显著上调(P＜0.05);Western blot结果也显示,Bax和Bcl-2的蛋白表达量也均发生了与mRNA相似的变化趋势(P＜0.05).结果表明,运输应激可通过调控Bax和Bcl-2的表达而促进山羊胃壁组织细胞发生凋亡.本研究可为改善运输应激对山羊器官组织损伤的方法提供理论依据.
Abstract
With the development of goat(Capra hircus)breeding,transportation-induced health problems in goats cannot be ignored.The aim of this study was to investigate the effects of transport stress on cell apoptosis and the expression of apoptosis-related genes,such as B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X protein(Bax)in gastric wall tissue of Ganxi goat.Twelve healthy one-year-old Ganxi male goats were randomly divided into control group(n=4),2 h transport group(n=4)and 6 h transport group(n=4).During the transportation process,food and water restrictions were carried out.After the transportation,tissues from stomach wall of the goats were collected and analyzed for relevant histology and quantification.Terminal dexynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL)results showed that the cell apoptotic rate of each stomach wall tissue in the 2 h transport group and the 6 h transport group was significantly increased(P＜0.05)compared to the control group.Additionally,the cell apoptotic rate of other gastric wall tissues,except for omasum,was obviously higher in the 6 h transport group than in the 2 h transport group(P＜0.05).Immunohistochemical results showed that Bax and Bcl-2 were mainly expressed in the mucosa epithelium of the anterior stomach(rumen,reticulum and omasum)and in the lamina propria of the abomasum.Bax expression in the 2 transport groups was clearly higher in the aforementioned areas of the stomach wall,with the decrease of Bcl-2 expression,but which was not obvious down-regulated in omasum.qPCR analysis revealed that the mRNA expression level of Bax was significantly up-regulated in other gastric wall tissues,except for omasum,in the 2 transport groups(P＜0.05),whereas the Bcl-2 mRNA expression was significantly down-regulated(P＜0.05).More importantly,Bax/Bcl-2 ratio in mRNA levels was significantly increased after transportation(P＜0.05).Western blot results also showed that there was a similar pattern in the Bax and Bcl-2 protein expression to that of mRNA.These findings indicated that transport stress could lead to cell apoptosis in goat stomach wall tissue through regulating the expression of Bax and Bcl-2.This work may be able to serve as a theoretical foundation for investigating strategies to reduce the damage of goat organs and tissues caused by transport stress.
关键词
山羊/运输应激/胃壁组织/细胞凋亡/B细胞淋巴瘤-2(Bcl-2)/Bcl-2相关X蛋白(Bax)Key words
Goat/Transport stress/Gastric wall tissues/Apoptosis/B-cell lymphoma-2(Bcl-2)/Bcl-2 associated X protein(Bax)引用本文复制引用
基金项目
江西省教育厅科技项目(GJJ211630)
江西省教育厅科技项目(GJJ201622)
江西省现代农业科研协同创新专项(JXXTCX201702)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据