梯棱羊肚菌促生恶臭假单胞菌X14的分离鉴定
Isolation and Identification of Morchella importuna Growth-promoting Pseudomonas putida X14
雷潇 1周冰玉 1周宇 1唐少军 1杨迪 2任锐 1马文杰 3马文华 3邵晨霞 1杨祎 1许隽1
作者信息
- 1. 湖南省微生物研究院,长沙 410009
- 2. 益阳欣博农业发展有限公司,益阳 413062
- 3. 常德市永春堂生物科技有限公司,常德 415010
- 折叠
摘要
羊肚菌(Morchella spp.)是全球著名的珍稀食药用菌,羊肚菌在人工栽培过程中必须覆土才能出菇,目前土壤微生物影响羊肚菌生长发育的机理仍未知.本研究从梯棱羊肚菌(Morchella importuna)菌基土壤中分离得到132株细菌,平板对峙试验发现有15株与梯棱羊肚菌菌丝存在拮抗作用,4株有促生作用.其中菌株X14与羊肚菌平板共培养4和8d,羊肚菌菌丝生长速率比对照分别显著增加7.6%和11.6%(P＜0.05);将羊肚菌和菌株X14接种于麦粒试管中共培养,在不同培养时间测定羊肚菌的菌丝生长速率.结果显示,培养4、6、7 d时,菌株X14处理的羊肚菌菌丝生长速率分别比对照极显著提高28.3%、24.8%、24.0%(P＜0.001).将羊肚菌和菌株X14接种于原种瓶中共培养,结果显示,共培养5d时,菌株X14处理的羊肚菌菌丝生长速率与对照无显著差异(P＞0.05),共培养10d时,菌株X14处理的羊肚菌菌丝生长速率比对照极显著提高14.1%(P＜0.01),原种培养周期比对照缩短4～6 d.综合菌落形态学特征、生理生化特征和16S rDNA序列分析结果,菌株X14被鉴定为恶臭假单胞菌(Pseudomonas putida).本研究从梯棱羊肚菌菌基土壤中筛选出1株恶臭假单胞菌X14能促进梯棱羊肚菌菌丝生长,为羊肚菌促生微生物菌剂的研发提供技术支持.
Abstract
Morel(Morchella spp.)is a world-famous rare edible and medicinal mushroom.In the artificial cultivation process morels must be covered with soil to produce fruit.At present,the mechanism of soil microorganisms affecting the growth and development of morel is still unknown.132 strains of bacteria were isolated and purified from morel soil.Through the plate confrontation culture test,it was found that 15 strains had an antagonistic effect on the hyphae of Morchella importuna and 4 strains had the effect of promoting growth.Among them,when strain X14 was co-cultured with morel on plate for 4 and 8 d,the growth rate of morel hyphae was significantly increased by 7.6%and 11.6%compared with the control(P＜0.05).Morel and strain X14 were inoculated into wheat medium in test tubes and co-cultured.The growth rate of morel hyphae was measured at different culture time.The results showed that the growth rate of morel hyphae treated by strain X14 was very extremely significantly increased by 28.3%,24.8%and 24.0%compared with the control at 4,6 and 7 d,respectively(P＜0.001).Morel and strain X14 were inoculated into stock flasks and co-cultured.The results showed that the growth rate of morel hyphae treated by strain X14 was not significantly different from the control at 5 d of co-culture(P＞0.05).After 10 d of co-cultivation,the growth rate of morel hyphae treated with strain X14 was extremely significantly increased by 14.1%compared with the control(P＜0.01).The culture period of the stock seed was shortened by 4～6 d compared with the control.Based on the comprehensive analysis of colony morphology,physiological and biochemical characteristics and 16S rDNA sequence,the strain X14 was identified as Pseudomonas putida.In this study,a strain of Pseudomonas putida X14 was screened from the soil of morel,which could promote the hyphae growth of Morchella importuna and provide technical support for the development of microbial agents that promote the growth of morel.
关键词
梯棱羊肚菌/恶臭假单胞菌/菌丝促生/微生物筛选Key words
Morchella importuna/Pseudomonas putida/Mycelial growth-promoting/Screening of microorganisms引用本文复制引用
基金项目
湖南省自然科学基金(2022JJ40233)
益阳市科技计划项目(2022108)
出版年
2024
国家科技期刊平台
NSTL
万方数据