牦牛妊娠早、中期胎盘组织中STAB1的表达、分布及功能分析
Expression,Distribution and Function Analysis of STAB1 in the Placenta of Yak(Bos grunniens)Early and Middle Pregnancy Phase
魏倩倩 1张全伟 2张博皓 3白旭 1赵兴绪 2张勇2
作者信息
- 1. 甘肃农业大学生命科学技术学院,兰州 730070;甘肃省动物生殖生理及繁殖调控重点实验室,兰州 730070
- 2. 甘肃农业大学生命科学技术学院,兰州 730070;甘肃农业大学动物医学院,兰州 730070;甘肃省动物生殖生理及繁殖调控重点实验室,兰州 730070
- 3. 甘肃农业大学动物医学院,兰州 730070;甘肃省动物生殖生理及繁殖调控重点实验室,兰州 730070
- 折叠
摘要
哺乳动物胎盘发育对子宫内环境调节及妊娠维持至关重要.胎盘发育不良或异常可引发多种妊娠疾病.稳定素蛋白-1(stabilin-1,STAB1)参与调节动物多个器官发育,但在牦牛(Bos grunniens)胎盘中的作用及调节机制尚不完全清楚.本研究旨在探究牦牛妊娠早、中期胎盘组织中STAB1分布、表达规律及其潜在功能.采集牦牛妊娠早、中期胎盘组织,通过苏木精-伊红(hematoxylin-eosin,HE)染色、免疫组织化学(immunohistochemistry,IHC)染色、免疫荧光(immunofluorescence,IF)染色、qRT-PCR和Western blot研究牦牛妊娠早、中期胎盘组织中STAB1表达特点及分布规律;利用数据非依赖性模式(data-independent acquisition,DIA)蛋白质组学数据,预测分析牦牛胎盘中STAB1互作蛋白及潜在生物学功能.结果表明,STAB1蛋白主要定位于牦牛胎盘滋养层巨细胞和单核滋养层细胞胞质;与妊娠早期胎盘相比,牦牛妊娠中期胎盘组织中STAB1基因及蛋白表达显著下调(P＜0.05);生物信息学分析显示,STAB1可能通过细胞黏附、钙离子结合和质膜组成等途径调控牦牛妊娠早、中期胎盘动态发育.综上,STAB1通过调节牦牛胎盘滋养层细胞的功能参与妊娠早、中期胎盘发育和妊娠维持.本研究为STAB1在牦牛胎盘发育中的功能及机制提供参考.
Abstract
The development of mammalian placenta is crucial for regulation of intrauterine environment and pregnant maintenance.Dysplasia or abnormality placenta could cause a variety of pregnant complications.Stabilin-1(STAB1)is involved in regulating the development of several organs in animals,but its function and regulatory mechanisms are not clearly understood in yak(Bos grunniens)placenta.This study aimed to investigate the distribution,expression pattern and potential function of STAB1 in yak placenta during early and middle pregnancy.Yak placental tissues were collected at early and middle pregnancy.The distribution and expression pattern of STAB1 in yak placental tissues during early and middle pregnancy were observed using hematoxylin-eosin(HE)staining,immunohistochemistry(IHC)staining,immunofluorescence(IF)staining,qRT-PCR and Western blot.Meanwhile,the interacting proteins and potential functions of STAB1 were predicted based on data-independent acquisition(DIA)proteomics data.The results showed that STAB1 protein was mainly localized in the cytoplasm of yak placenta trophoblast giant cells and uninucleate trophoblast cells.Compared to early pregnancy placenta,the expression of STAB1 gene and its protein levels were significantly down-regulated in yak middle pregnancy(P＜0.05).Bioinformatics analysis revealed that STAB1 might regulate cell adhesion,calcium binding and plasma membrane composition and other pathways involved in the dynamic development of yak's placenta during the early and middle pregnancy.In conclusion,STAB1 might be involved in placental development and pregnancy maintenance in early and middle pregnancy through regulating the function of placental trophoblast cells in yaks.This study provides a reference for further investigation of the function and mechanism of STAB1 in yak placental growth and development.
关键词
胎盘/稳定素蛋白-1(STAB1)/DIA蛋白质组学/生物信息学分析Key words
Placenta/Stabilin-1(STAB1)/Data-independent acquisition(DIA)proteomics/Bioinformatics analysis引用本文复制引用
基金项目
中科院"西部之光"(03321013)
甘肃省自然科学基金重点项目西部之光项目配套(21JR7RA799)
国家自然科学基金(32360836)
出版年
2024
国家科技期刊平台
NSTL
万方数据