贝莱斯芽胞杆菌YFB3-1对辣椒疫霉的抑菌效果及其抑菌物质
Antibacterial Effect of Bacillus velezensis YFB3-1 on Phytophthora capsici and Its Antibacterial Substances
穆晓清 1鲁耀雄 2戴良英 1黄国林 3陈薇 4张榉邺 4韦晨曦4
作者信息
- 1. 湖南农业大学植物保护学院,长沙 410128
- 2. 湖南农业大学植物保护学院,长沙 410128;湖南省农业科学院农业环境生态研究所,长沙 410125
- 3. 湖南省农业科学院园艺研究所,长沙 410125
- 4. 湖南农业大学园艺学院,长沙 410128
- 折叠
摘要
辣椒疫病是由疫霉菌(Phytophthora capsic)侵染造成辣椒(Capsicum annuum)毁灭性损失的土传病害,严重威胁我国辣椒产业的可持续发展.为了获得安全高效、有效预防辣椒疫病的生防制剂,本研究选取从蚯蚓(Eisenia foetida)肠道细菌中分离鉴定的一株贝莱斯芽胞杆菌(Bacillus velezensis)YFB3-1为生防菌株,以辣椒疫霉菌为靶标菌,通过平板对峙法比较不同种类的芽胞杆菌对辣椒疫霉菌的抑菌效果,及YFB3-1对辣椒不同土传病原真菌的抑菌效果,并分析其主要的抑菌次级代谢物和生防机制.抑菌试验表明,贝莱斯芽胞杆菌YFB3-1对辣椒疫霉菌的抑制效果最好,抑菌率达85.21%,比枯草芽胞杆菌(Bacillus subtilis)GCK5-1对辣椒疫霉菌的抑菌率提高了19.8%,对辣椒不同土传病原真菌的抑菌效果较好,具有广谱抗辣椒土传病原真菌的特性.显微镜镜检表明,贝莱斯芽胞杆菌YFB3-1能够导致疫霉菌菌丝生长异常,多分枝或分枝杂乱,菌丝细胞不同程度地扭曲和膨大,甚至细胞壁破裂;液相色谱串联质谱(liquid chromatography tandem mass spectrometry,LC-MS)靶向代谢组测定结果显示,贝莱斯芽胞杆菌YFB3-1发酵液中的主要脂肽类化合物为表面活性素(surfactin)和丰原素(fengycin),在发酵3d达最高含量,分别为1.19×10-3和1.14×10-3 mg/mL.本研究为辣椒疫病的绿色防控提供了优异的生防菌株,为进一步挖掘利用贝莱斯芽胞杆菌的次级代谢物质提供了参考依据.
Abstract
Pepper phytophthora blight is a soil-borne disease caused by Phytophthora capsic,which causes devastating losses of pepper(Capsicum annuum)and seriously threatens the sustainable development of pepper industry in China.In order to obtain a safe,efficient and effective biocontrol agent for the prevention of pepper phytophthora blight,a strain of Bacillus velezensis YFB3-1 isolated and identified from the intestinal bacteria of earthworms(Eisenia foetida)was selected as a biocontrol strain,and Phytophthora capsici was used as the target strain.The antibacterial effects of different types of Bacillus on P.capsici were compared by plate confrontation method,and the antibacterial effects of YFB3-1 on different soil-borne pathogenic fungi of pepper were compared,and the main antibacterial secondary metabolites and biocontrol mechanisms were analyzed.The antibacterial test showed that B.velezensis YFB3-1 had the best inhibitory effect on P.capsici,and the inhibition rate was 85.21%,which was 19.8%higher than that of B.subtilis GCK5-1.It had good antibacterial effect on different soil-borne pathogenic fungi of pepper,and had the characteristics of broad-spectrum resistance to soil-borne pathogenic fungi of pepper.Microscopic examination showed that B.velezensis YFB3-1 could lead to abnormal mycelial growth,multi-branching or disordered branches,resulting in distortion and enlargement of mycelial cells to varying degrees,and even cell wall rupture.The results of liquid chromatography tandem mass spectrometry(LC-MS)targeted metabolomics showed that the main lipopeptide compounds in the fermentation broth of B.velezensis YFB3-1 were surfactin and fengycin,which reached the highest content at 3 d of fermentation,which were 1.19×10-3 and 1.14×10-3 mg/mL,respectively.This study provides an excellent biocontrol strain for the green prevention and control of pepper blight,and provides a reference for further excavation and utilization of secondary metabolites of B.velezensis.
关键词
贝莱斯芽胞杆菌/辣椒疫霉/抑菌效果/次级代谢物/脂肽类化合物Key words
Bacillus velezensis/Phytophthora capsici/Antibacterial effect/Secondary metabolite/Lipopeptide引用本文复制引用
基金项目
湖南省重点研发计划(2021NK2008)
湖南省重点研发计划(2022NK2062)
湖南省自然科学基金(2020JJ4393)
长沙市自然科学基金(kq2208105)
出版年
2024
国家科技期刊平台
NSTL
万方数据