Responses of SiPHYA and SiPHYB Gene to Light Quality,Photoperiod and Abiotic Stresses in Foxtail Millet(Setaria italica)
Phytochromes are an red/far-red receptors that regulate seed germination,plant height,flowering,and shade avoidance response in plants.To explore the response patterns of photochrome genes(SiPHYA and SiPHYB)to light quality,photoperiod and abiotic stress in foxtail millet,The cDNA sequences of SiPHYA(Seita.9G113600)and SiPHYB(Seita.9G427800)were cloned by RT-PCR,and the results showed that the full length of SiPHYA coding region was 3 396 bp,encoding 1 131 amino acids,and SiPHYB was 3 507 bp,encoding 1 169 amino acids.Phylogenetic trees of multi-species were constructed by MEGA-X,which indicated that SiPHYA and SiPHYB had a closer evolutionary relationship to ZmPHYA and ZmPHYB,respectively.Transcription factors binding to the promoters of the photochrome genes were predicted via the online website PlantRegMap,the results showed that 109 transcription factor from 29 families could bind to the promoter region of SiPHYA,and 93 transcription factor from 27 families could bind to the promoter region of SiPHYB.Furthermore,the expression patterns in different tissues and organs,light quality treatments,photoperiod treatments and stress treatments were analysed using qRT-PCR.qRT-PCR results showed that SiPHYA and SiPHYB were expressed in roots,stems,leaves and young panicles,with the highest expression levels in the flag leaves.The response patterns of SiPHYA and SiPHYB were diverse under different light quality treatment between'Henggu12'and'Changnong35',of which,SiPHYA had the highest expression level under darkness in'Henggu12',while SiPHYA had the highest expression level under far-red light in'Changnong35',and SiPHYB had the highest expression level under white light,and lowest expression under red light in both'Henggu12'and'Changnong35'.Under long-day,SiPHYA showed similar circadian rhythm changes in both'Henggu12'and'Changnong35',while SiPHYB showed rhythmic changes in'Changnong35',but no rhythmic changes in'Henggu12'.Under short-day,SiPHYA and SiPHYB showed no circadian rhythm changes in both'Henggu12'and'Changnong35'.Both SiPHYA and SiPHYB were responsive to high salt,high temperature,low temperature and drought stresses,but the response patterns were diverse.This study provides clues for furher exploring the mechanisms of SiPHYA and SiPHYB in the development and abiotic stresses,and provides a basis for their application in the genetic improvement of foxtai millet.
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