单细胞转录组测序技术及其在动物骨骼研究中的应用
Single-cell RNA Sequencing Technology and Its Application in Animal Bone Research
刘哲 1田学凯 1何玉琳 1杨培钰 1于太永1
作者信息
- 1. 西北农林科技大学动物科技学院,杨凌 712100
- 折叠
摘要
骨骼是动物机体重要部分,在提供生物力学支持、维持运动、造血和钙稳态等方面发挥重要作用.普通转录组测序(bulk RNA sequencing,bulk RNA-seq)在骨骼研究中的应用已为骨细胞的发育研究提供了重要参考,但仍有局限性,无法揭示骨骼细胞的异质性.近年来兴起的单细胞转录组测序(single-cell RNA sequencing,scRNA-seq)技术在细胞捕获效率和存活率等方面都得到了极大的提高,研究人员可以在群体细胞中以单个细胞的角度解读生物学信息,为研究骨骼细胞间异质性信息和细胞群体之间的关系提供了新的研究策略.本文就单细胞转录组测序技术及其在动物骨骼研究中的应用进展进行综述,以期为后续的研究提供参考.
Abstract
The skeleton is an important part of the animal body,playing a vital role in providing biomechanical support,maintaining movement,hematopoiesis,and calcium homeostasis.The application of bulk RNA sequencing(bulk RNA-seq)technology in skeletal research has provided important references for revealing the development of bone cells,but it still has limitations in revealing the heterogeneity of bone cells.In recent years,the emerging single-cell RNA sequencing(scRNA-seq)technology has greatly improved the efficiency of cell capture and survival rate,allowing researchers to interpret biological information from the perspective of individual cells in a population of cells,providing a new research strategy for studying the heterogeneity of bone cells and the relationship between cell populations.This article reviewed the progress of single-cell RNA sequencing technology and its application in animal bone research,with the aim of providing reference for future studies.
关键词
单细胞转录组测序(scRNA-seq)/动物骨骼/细胞异质性Key words
Single-cell RNA sequencing(scRNA-seq)/Animal bones/Cellular heterogeneity引用本文复制引用
基金项目
国家重点研发计划(2021YFD1301205)
出版年
2024
国家科技期刊平台
NSTL
万方数据