小麦TaPR1基因启动子分析及其调控因子的筛选
Analysis of Wheat(Triticum aestivum)TaPR1 Gene Promoter and Screening of Its Regulators
申松松 1王丽珊 1冯燕 1崔钟池 1刘园霞 1刘大群 1王海燕1
作者信息
- 1. 河北农业大学植物保护学院/河北省农作物病虫害生物防治技术创新中心,保定 071001
- 折叠
摘要
病程相关蛋白1(pathogenesis-related protein 1,PR1)是植物重要的抗逆防御蛋白,小麦(Triticum aestivum)TaPR1基因受小麦叶锈菌(Puccinia triticina,Pt)诱导表达,参与小麦对叶锈病的防御反应.本课题组前期利用基因克隆方法成功获得TaPR1启动子序列,命名为pTaPR1,其中900 bp序列(pTaPR1900)具有较强的活性.本研究进一步对pTaPR1的序列组成和可能的调控因子进行分析.根据PLACE和PlantCARE数据库初步分析,pTaPR1序列中包含核心元件TATA-box、CAAT-box及大量光反应元件,还包含脱落酸应答元件和响应低温诱导的MYB结合位点.利用酵母单杂交技术(yeast one-hybrid,Y1H)筛选,共获得12个可能与pTaPR1900结合的转录因子;利用Y1H回复验证,初步证实U-box E3泛素连接酶、花粉蛋白、甘氨酸A3蛋白和重金属伴生异戊二烯基植物蛋白与pTaPR1900互作.本研究为深入探讨TaPR1转录调控机制提供理论参考.
Abstract
Pathogenesis-related protein 1(PR1)is an important defense protein in plants,its production and accumulation in plants is one of the important responses to biotic and abiotic stresses.In previous study,TaPR1 was found to be induced by Puccinia triticina(Pt)and participate in wheat defense response to leaf rust.In the early stage of this study,the TaPR1 promoter sequence was successfully obtained by gene cloning and named pTaPR1,among which the 900 bp sequence(pTaPR1900)had strong activity.This study further analyzed the sequence composition and possible regulatory factors of pTaPR1.Preliminary analysis based on the PLACE and PlantCARE databases showed that pTaPR1 contained the core elements including TATA-box,CAAT-box and a large number of light-responsive elements,as well as the ABA-responsive element and the MYB-binding site.Yeast one-hybrid(Y1H)technique was used to screen the transcription factors that might bind to pTaPR1900 involved in regulating TaPR1 gene expression,12 candidates were obtained.The interaction between 4 candidates including U-box E3 ubiquitin ligase,pollen protein,glycine A3 protein and heavy metal-associated isoprenoid phycobiliprotein with pTaPR1900 were initially verified.Taken together,this study provides theoretical reference for exploring the transcriptional regulatory mechanism of TaPR1.
关键词
小麦病程相关蛋白1(TaPR1)/启动子/顺式作用元件/酵母单杂交(Y1H)/转录因子Key words
Triticum aestivum pathogenesis-related protein 1(TaPR1)/Promoter/Cis-acting element/Yeast one-hybrid(Y1H)/Transcription factor引用本文复制引用
基金项目
国家自然科学基金(32172384)
国家自然科学基金(31501623)
出版年
2024
国家科技期刊平台
NSTL
万方数据