植物病原真菌m6A识别蛋白的挖掘与功能分析
Mining and Functional Analysis of m6A Recognition Proteins of Plant Pathogenic Fungi
何旭 1朱蒙芳 1董彩奕 1王炫 1杨梦雨 1巩校东 1魏淑珍 2谷守芹 1刘玉卫 1贾慧1
作者信息
- 1. 河北农业大学生命科学学院/河北省农业微生物生物信息利用技术创新中心/华北作物改良与调控国家重点实验室,保定 071000
- 2. 河北省湿地保护与绿色发展协同创新中心,衡水 053000
- 折叠
摘要
N6-甲基腺嘌呤(N6-methyladenosine,m6A)修饰是真核生物中最常见的RNA修饰.包含YT521-B同源(YT521-B homology,YTH)结构域的蛋白可识别m6A修饰,从而介导RNA代谢.该类蛋白在植物病原真菌中的研究还未见报道.本研究利用生物信息学方法,对127种植物病原真菌中的m6A识别蛋白进行了系统鉴定和分析,并对该类蛋白在玉米大斑病菌(Setosphaeria turcica)发育过程中的基因表达模式进行分析.结果发现,在供试的127个菌株中共鉴定了225个m6A识别蛋白,其中担子菌门中93%真菌含有2个m6A识别蛋白;理化性质分析发现,在鉴定的225个m6A识别蛋白中,有67.56%的蛋白呈碱性,且不同门及不同生活方式真菌的m6A识别蛋白酸碱性存在显著差异.系统发育分析发现,225个m6A识别蛋白可分为5组,其中2组与人类(Homo sapiens)具有较近的进化关系,相同门和相同生活方式真菌的m6A识别蛋白倾向于聚类在同一进化分支上;保守位点分析发现,YTH结构域序列在所有鉴定的m6A识别蛋白中存在多个保守型位点.在玉米大斑病菌中鉴定了1个编码该类蛋白的基因StYTH1,表达量分析发现,其在病菌附着胞发育时期的表达量最高,可能对病菌附着胞发育具有重要的调控作用.本研究为深入揭示m6A识别蛋白的功能提供了基础资料.
Abstract
The N6-methyladenosine(m6A)is the most common RNA modification in eukaryotes.Proteins containing the YT521-B(YTH)domain recognize the m6A site and mediate RNA metabolism.However,the function of m6A recognition proteins in plant pathogenic fungi has not been reported yet.This study systematically identified and analyzed the m6A recognition proteins in 127 plant pathogenic fungi using bioinformatics method.Furthermore,the expression patterns of the genes encoding m6A recognition proteins during the development of Setosphaeria turcica were analyzed.The results showed that a total of 225 m6A recognition proteins were identified in the tested 127 fungi,and 93%of the fungi in the Basidiomycota phylum had 2 m6A recognition proteins.Physicochemical analysis showed that 67.56%of the m6A recognized proteins identified were alkaline.Notably,significant differences in the acidity and alkalinity of these proteins were observed within different phyla and fungal lifestyles.Phylogenetic analysis revealed that 225 m6A recognition proteins could be classified into 5 groups,2 of which shared a closer evolutionary relationship with humans(Homo sapiens).Additionally,m6A recognition proteins from fungi with the same phylum and lifestyle tended to cluster together on evolutionary branches.The conserved site analysis uncovered the existence of several conserved-type sites in the sequences of the YTH domain across the 225 m6A recognized proteins.A gene that encodes the m6A recognition protein,namely StYTH1,was identified in S.turcica.Expression analysis revealed that StYTH1 had the highest expression level in the developmental stage of appressorium,suggesting that StYTH1 might be involved in regulating the development of appressorium.The present study provides basic materials for an insightful revelation of the functions of the m6A recognition proteins.
关键词
植物病原真菌/N6-甲基腺嘌呤(m6A)识别蛋白/生物信息学分析/表达模式Key words
Plant pathogenic fungi/N6-methyladenosine(m6A)recognition protein/Bioinformatics analysis/Expression pattern引用本文复制引用
基金项目
中央引导地方科技发展资金项目(216Z2902G)
中央引导地方科技发展资金项目(236Z6507G)
河北省高等学校基础科研经费项目(KY2022037)
河北省高等学校基础科研经费项目(KY2021042)
河北省自然科学基金(C2020204183)
出版年
2024
国家科技期刊平台
NSTL
万方数据