辣椒根际促生菌的筛选及促生效应分析
Screening of Rhizosphere Growth-promoting Bacteria of Pepper(Capsicum annuum)and Analysis of Their Beneficial Effects
张琳 1余红凤 1毕钰 1王志刚 1徐伟慧 1刘桂琳1
作者信息
- 1. 齐齐哈尔大学生命科学与农林学院/黑龙江省农业微生物制剂产业化技术创新中心/黑龙江省农用生物制剂产业化协同创新中心,齐齐哈尔 161006
- 折叠
摘要
过度施用化肥农药以及土壤连作问题严重制约了辣椒的生产,因此改良土壤环境,对提高辣椒(Capsicum annuum)产量十分重要.为了筛选辣椒根际促生菌并研究其促生效应,本研究以齐齐哈尔市辣椒根际土壤为材料,使用功能性培养基分离筛选辣椒根际中的促生菌,通过16S rDNA序列对菌株进行鉴定,采用种子萌发试验以及盆栽试验验证促生能力强的菌株的促生效果.结果表明,共分离出51株辣椒根际促生菌,分属于12个菌属.51株促生菌中有8株菌具有解磷能力,35株菌能产赤霉素,33株菌能产生长素(auxin,IAA),所有菌株均具有溶磷、解钾和固氮的能力.其中特基拉芽胞杆菌(Bacillus tequilensis)K10能够显著促进辣椒种子萌发,并增加辣椒幼根根毛的数量.暹罗芽胞杆菌(B.siamensis)G19-1和约氏不动杆菌(Acinetobacter johnsonii)N9-2能够显著增加辣椒植株的地上部鲜重、根鲜重、地上部干重、根干重以及株高(P＜0.05),且菌株的溶磷、解钾、固氮、产赤霉素和IAA能力与辣椒植株地上鲜重、地上干重以及根干重之间呈显著正相关(P＜0.05).综上,本研究共筛选得到的3株促生菌K10、G19-1和N9-2可作为辣椒微生物肥料的候选菌株,为辣椒微生物制剂的开发提供菌种资源与技术支持.
Abstract
Excessive application of chemical fertilizers and pesticides and soil continuous cropping have seriously restricted the production of pepper(Capsicum annuum),so it is very important to improve the soil environment so as to increase the yield of pepper.In order to screen rhizosphere growth-promoting bacteria of pepper and study their promoting effect,the rhizosphere soil from pepper in Qiqihar city was used as experimental materials.The growth-promoting bacteria in the rhizosphere of pepper was screened out using functional media,and identified by 16S rDNA sequence.The seed germination and pot experiments were conducted to verify the growth-promoting effect of the strains with strong growth-promoting ability.A total of 51 strains of pepper rhizosphere growth-promoting bacteria were isolated,which belonged to 12 genera.The results showed that among the 51 growth-promoting bacteria,8 strains had the ability of phosphorus resolving,35 strains could produce gibberellin,33 strains could secrete auxin(IAA),and all 51 strains had the ability of phosphorus-solubilizing,potassium releasing and nitrogen fixation.Bacillus tequilensis K10 could significantly promote pepper seed germination and increased the number of root hairs of pepper young roots.B.siamensis G19-1 and Acinetobacter johnsonii N9-2 significantly increased above ground fresh weight,root fresh weight,aboveground dry weight,root dry weight and plant height(P＜0.05),and the ability of bacteria to solubilize phosphorus,release potassium,fix nitrogen,produce gibberellins and IAA was significantly positively correlated with aboveground fresh weight,aboveground dry weight,and root dry weight of pepper plants(P＜0.05).In conclusion,this experiment screened 3 growth-promoting bacteria K10,G19-1 and N9-2 as candidate strains for pepper microbial fertilizer,and will provide strain resources and technical support for the development of pepper microbial fertilizer.
关键词
辣椒/分离/促生效应/根际促生菌Key words
Capsicum annuum/Isolation/Growth promoting effect/Plant growth promoting rhizobacteria引用本文复制引用
基金项目
齐齐哈尔市食品产业研究院项目(SXSP-2021004)
黑龙江省属本科高校基本科研业务费成果转化后补助项目(145209808)
出版年
2024
国家科技期刊平台
NSTL
万方数据