贝莱斯芽胞杆菌WB对西瓜植株的促生效应和机制
Promoting Effects and Mechanism of Bacillus velezensis WB on Watermelon(Citrullus lanatus)Plants
陈忠男 1王志刚 1徐伟慧1
作者信息
- 1. 齐齐哈尔大学生命科学与农林学院,齐齐哈尔 161006;黑龙江省农业微生物制剂产业化技术创新中心,齐齐哈尔 161006;黑龙江省农用生物制剂产业化协同创新中心,齐齐哈尔 161006
- 折叠
摘要
西瓜(Citrullus lanatus)是最受欢迎的水果之一,随着人们对生活品质的不断追求,提高产品质量已成为农作物高销量的关键因素.植物根际促生菌是一类能够促进植物吸收土壤中营养元素并提高植物健康的有益细菌.为了探究贝莱斯芽胞杆菌(Bacillus velezensis)菌株WB对西瓜植株的促生效应.本研究以菌株WB和西瓜种子为材料,使用功能性培养基解析菌株WB的促生能力,采用种子萌发实验以及盆栽实验验证菌株WB的促生效应并利用转录组测序(RNA sequencing,RNA-seq)技术阐释菌株WB的促生机制.结果表明,贝莱斯芽胞杆菌WB具有产生长素(indole-acetic acid,IAA)与纤维素酶的能力,还具有解磷、解钾和固氮的能力.种子萌发实验和盆栽实验的结果表明,菌株WB对西瓜植株的生长有促进作用.此外,菌株WB上调了与植物促生长相关的信号通路中基因的表达,包括:半萜和三萜类生物合成、苯丙烷类生物合成和植物激素信号转导途径;参与促进生长的转录因子,如MYB(myeloblastosis)、NAC(NAM,ATAF,CUC)和Dof(DNA binding with one finger)等也被诱导上调表达.综上,本研究发现了贝莱斯芽胞杆菌WB可以促进西瓜生长并对其促生机制进行了阐释,为其进一步应用提供了理论依据.
Abstract
Watermelon(Citrullus lanatus)is one of the most popular fruits.With people's continuous pursuit of quality of life,improving products quality has become a key factor in high sales of crops.Plant growth promoting rhizobacteria are a group of beneficial bacteria that promote plant uptake of nutrients from the soil and improve plant health.To investigate the growth promoting effect of Bacillus velezensis WB on watermelon plants.Strain WB and watermelon seeds were selected as materials,the growth promoting ability of strain WB was analyzed using functional culture medium,the growth promoting effect of strain WB was validated using seed germination experiments and pot experiments,and the growth promoting mechanism of strain WB was explored using RNA sequencing(RNA-seq).The results showed that B.velezensis WB had the ability to produce auxin(IAA)and cellulase,as well as the ability to solubilize phosphorus,potassium,and to fixate nitrogen.The results of seed germination and pot experiments indicated that strain WB had a promoting effect on the growth of watermelon plants.In addition,strain WB upregulated the expression of genes related to plant growth promoting signaling pathways,including sesquiterpenoid and triterpenoid biosynthesis,phenylpropanoid biosynthesis,and plant hormone signal transduction pathway.Transcription factors were expressed in promoting growth,such as myoptosis(MYB),NAC(NAM,ATAF and CUC),and Dof(DNA binding with one finger).In conclusion,this study found that B.velezensis WB could promote the growth of watermelon and elucidated its growth promoting mechanisms,and providing theoretical basis for its application in future.
关键词
芽胞杆菌/西瓜/促进生长/转录组测序(RNA-seq)Key words
Bacillus velezensis/Watermelon/Growth-promoting/RNA sequencing(RNA-seq)引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据