白羽王鸽CAPN11基因SNPs鉴定及其对屠宰性状的影响
Identification of CAPN11 Gene SNPs in White King Pigeons(Columba livia)and Their Impact on Slaughter Traits
向进 1龙霞 1庞金兰 2成敏 3张依裕 1杨阿明2
作者信息
- 1. 贵州大学动物科学学院/高原山地动物遗传育种与繁殖重点实验室/贵州省动物遗传育种与繁殖重点实验室,贵阳 550025
- 2. 黔西市农业农村局,黔西 551500
- 3. 贵州裕黔农业生物科技发展有限公司,黔西 551500
- 折叠
摘要
白羽王鸽(Columba livia)是重要的经济禽类,探究其钙蛋白酶11(calpain 11,CAPN11)基因多态性与屠宰性状的关系,对其屠宰性状的遗传改良和经济性状提升具有重要意义.本研究采用PCR直接测序法对白羽王鸽CAPN11基因SNP进行筛查,并分析SNP位点与白羽王鸽屠宰性状的关联性.通过鉴定白羽王鸽的CAPN11 基因的SNPs发现,在外显子 6 区的第 636 和 738 位发现g.3641644 C>T和g.3641746 C>T 2个同义突变,均存在3种基因型,优势基因型和优势等位基因均为CT和C,0.25＜PIC＜0.5均呈中度多态;χ2检验显示,2个SNPs位点的基因型分布均符合Hardy-Weinberg平衡状态(P＞0.05);连锁不平衡分析显示,2个SNPs位点之间不满足D'＞0.80,r2＞0.33,表明不存在强连锁不平衡效应;关联性分析显示,g.3641644 C>T位点对宰前活重的影响达到显著水平(P＜0.05);g.3641746 C>T位点对白羽王鸽的宰前活重、屠体重、全净膛重、胸肌重和腿肌重等屠宰性状的影响均达到了显著水平(P＜0.05);双倍型分析发现,2个SNPs位点在实验群体中共检测到4种单倍型和10种双倍型,双倍型H2H2(CCTT)个体的宰前活重、屠体重、全净膛重和腿肌重均显著高于其他9种双倍型(P＜0.05);双倍型H2H2(CCTT)个体的胸肌重显著高于除双倍型H3H3(TTCC)和H3H4(TTCT)外的其他双倍型个体(P＜0.05).通过分析单倍型mRNA二级结构发现,单倍型H1(CC)、H2(CT)、H3(TC)均引起了CAPN11基因的mRNA二级结构变化,可能会影响转录和翻译效率,进而影响屠宰性能.综上,CAPN11基因能够作为潜在候选基因用于改善白羽王鸽屠宰性状,为加速白羽王鸽育种选育进程提供一定的理论依据,为提升品种屠宰性状的分子标记选育工作提供参考.
Abstract
White king pigeon(Columba livia)is an important economic poultry in China.To explore the relationship between calpain 11(CAPN11)gene polymorphism and slaughtering traits is of great significance to the genetic improvement of slaughtering traits and the improvement of economic traits.In this study,SNP of CAPN11 gene of white king pigeon was screened by PCR direct sequencing method,and the relationship between SNP locus and slaughtering traits was analyzed.Through the identification of the SNPs of the CAPN11 gene of the white king pigeon,it was found that g.3641644C>T and g.3641746C>T 2 synonymous mutations were found at positions 636 and 738 in the exon 6 region of CAPN11 gene of the white king pigeon,all of which had 3 genotypes,the dominant genotypes and alleles were CT and C,0.25＜PIC＜0.5.χ2 test showed that the genotypic distribution of the two SNPs loci were in accordance with Hardy-Weinberg equilibrium.Linkage disequilibrium analysis showed that D'＞0.80 and r2＞0.33 were not satisfied between the 2 SNPs,indicating that there was no strong linkage disequilibrium effect.Correlation analysis showed that g.3641644C>T locus had a significant effect on antemortem live weight(P＜0.05).The effects of g.3641746C>T locus on pre-slaughter live weight,dressed weight,eviscerated weight,chest muscle weight and leg muscle weight of white king pigeons reached significant level(P＜0.05).Diplotype analysis showed that 4 haplotypes and 10 diplotypes were detected at 2 SNPs loci in the experimental population.The antemortem live weight,dressed weight,eviscerated weight and leg muscle weight of individuals with haplotype H2H2(CCTT)were significantly higher than those of the other 9 diplotypes,and the chest muscle weight of individuals with haplotype H2H2(CCTT)was significantly higher than that of individuals with other diplotypes except H3H3(TTCC)and H3H4(TTCT).Through the analysis of the secondary structure of haplotype mRNA,it was found that haplotypes H1(CC),H2(CT)and H3(TC)all caused changes in the mRNA secondary structure of CAPN11 gene,which might affect the efficiency of transcription and translation,and then affect the slaughtering performance.In summary,CAPN11 gene can be used as a potential candidate gene to improve molecular marker selection for slaughtering traits of white king pigeons.This study provides a theoretical basis for accelerating the breeding process of white king pigeons and reference for improving molecular marker breeding of slaughtering traits of white king pigeons.
关键词
白羽王鸽/钙蛋白酶11基因(CAPN11)/SNPs/屠宰性状Key words
White king pigeon/Calpain 11 gene(CAPN11)/SNP/Slaughter traits引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据