利用组蛋白去乙酰化酶抑制剂提高猪体细胞核移植胚胎发育率的研究进展
Research Progress on Improvement in Development of Porcine Somatic Cell Nuclear Transfer Embryos by Histone Deacetylase Inhibitors
晏超 1陈指龙 2彭翠婷 2谢浩 2张才用 2赵玉兰 3齐霖 2刘永刚 4唐中林5
作者信息
- 1. 云南农业大学动物科学技术学院,昆明 650201;中国农业科学院深圳农业基因组研究所,深圳 518000
- 2. 中国农业科学院深圳农业基因组研究所,深圳 518000
- 3. 佛山鲲鹏现代农业研究院,佛山 528226
- 4. 云南农业大学动物科学技术学院,昆明 650201
- 5. 中国农业科学院深圳农业基因组研究所,深圳 518000;佛山鲲鹏现代农业研究院,佛山 528226;岭南现代农业科学与技术广东省实验室深圳分中心,深圳 518000;农业农村部畜禽生物组学重点实验室,深圳 518000
- 折叠
摘要
体细胞核移植(somatic cell nuclear transfer,SCNT)技术在保护濒危物种、扩繁良种和治疗疾病等方面应用广泛.组蛋白乙酰化修饰是调控猪(Sus scrofa)早期胚胎发育重要的表观遗传标记,其正确的擦除与重建是胚胎正常发育的基础.随着微量细胞测序技术的发展,研究人员发现体细胞克隆胚胎重编程过程中存在多种表观遗传修饰异常,其中组蛋白乙酰化修饰异常是导致猪体细胞克隆胚胎发育阻滞的重要因素.近年来,一些组蛋白去乙酰化酶抑制剂逐渐被发掘,可用于改善胚胎发育过程中组蛋白乙酰化的异常修饰水平.本文综述了近年来利用组蛋白去乙酰化酶抑制剂提高猪体细胞核移植胚胎发育及其作用机制的相关研究,为提高猪体细胞克隆效率提供参考.
Abstract
Somatic cell nuclear transfer(SCNT)has been widely used in protecting endangered species,propagating better varieties and treating diseases.Histone acetylase is an important epigenetic mark regulating the development of early embryo in pig(Sus scrofa),and its correct obliteration and reconstruction are the basis of the embryonic development.With the development of low input next generation sequencing,many studies found that a variety of abnormal epigenetic modification have been found during the reprogramming of somatic cell nuclear transfer embryos.Histone acetylase modification is an important factor that leads to the development block of porcine somatic cell nuclear transfer embryos.In recent years,some small molecule compounds of histone deacetylase inhibitors have been gradually discovered for the improvement of abnormal modification level of histone acetylase during embryonic development.This paper reviews the studies of the effects and mechanisms of histone deacetylase inhibitors on the development of porcine somatic cell nuclear transfer embryos,which provides reference for improving the cloning efficiency in pig.
关键词
猪/体细胞核移植(SCNT)/组蛋白去乙酰化酶抑制剂/胚胎/发育Key words
Pig/Somatic cell nuclear transfer(SCNT)/Histone deacetylase inhibitors/Embryo/Development引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据