小麦WUS基因亚家族的全基因组鉴定及其在雄蕊和雌蕊中的表达分析
Genome-wide Identification and Expression Analysis of WUS Gene Subfamily in Wheat(Triticum aestivum)Pistil and Stamen
赵若男 1邹瑞 2YAMAMOTO Naoki 1陈真勇 1吴一超 1蒋进 3彭正松 4杨在君1
作者信息
- 1. 西华师范大学生命科学学院/西南野生动植物资源保护教育部重点实验室,南充 637000
- 2. 四川省阿坝州农产品质量检验监测中心,马尔康 624011
- 3. 南充市农业科学院,南充 637000
- 4. 西昌学院农业科学学院,西昌 615013
- 折叠
摘要
WUSCHEL(WUS)是一种同源结构域转录因子,其作为WOX家族的一个进化分支,可能通过调节特定靶基因的表达,在植物细胞的胚胎发生和芽再生中发挥重要作用.本研究利用生物信息学方法在小麦(Triticum aestivum)的全基因组中一共鉴定出25个WUS基因,不均匀分布在第1、第2、第3和第5同源群染色体上,并且其在第3同源群染色体上发生了串联重复现象.保守结构域分析显示,该家族成员均含有motif 1和motif 3,基因结构和进化树显示,聚类在一起的WUS蛋白具有类似的基因结构.在TaWUSs的启动子区共鉴定出18类激素响应与非生物胁迫类的响应元件.组织表达谱分析显示,25个TaWUSs在小麦中拥有明显的组织表达特异性,且多数在花器官高表达.qRT-PCR结果进一步证实了TaWUS-2A1、TaWUS-3B2、TaWUS-3D2、TaWUS-5A1、TaWUS-5B1 和 TaWUS-5D1 在正常雄蕊中高表达,而TaWUS-3D1在正常雌蕊中高表达.尤其是TaWUS-1A、TaWUS-1B、TaWUS-1D和TaWUS-2D1在雌蕊化雄蕊中高表达,预示其可能在调控小麦同源转化型雄性不育突变体(HTS-1)中雄蕊同源转化为雌蕊性状的形成中发挥作用.本研究为进一步解析小麦WUS亚家族基因的功能提供了理论基础.
Abstract
WUSCHEL(WUS)is a homeodomain transcription factor,an evolutionary branch of the WOX family,which may play an important role in embryogenesis and bud regeneration in plant cells by regulating the expression of specific target genes.In this study,a total of 25 WUS genes were identified in the entire genome of wheat(Triticum aestivum)by bioinformatics methods.These genes were distributed unevenly on the first,second,third and the fifth homologous group of chromosomes,with tandem duplication observed on the third homologous group chromosomes.Conserved domain and structural analysis showed that motif 1 and motif 3 were present in all members of the family,and the WUS proteins clustered together showed similar gene structure according to the evolutionary tree.A total of 18 hormone response and abiotic stress response elements were identified in the promoter region of TaWUSs.Tissue expression profile analysis showed that the 25 detected TaWUSs had obvious tissue expression specificity in wheat,and most of them showed high expression in the floral reproductive organs.qRT-PCR results also confirmed that TaWUS-2A1,TaWUS-3B2,TaWUS-3D2,TaWUS-5A1,TaWUS-5B1 and TaWUS-5D1 were highly expressed in stamens,and TaWUS-3D1 was highly expressed in pistils.Especially,the high expression of TaWUS-1A,TaWUS-1B,TaWUS-1D,and TaWUS-2D1 in pistillody stamens might indicate their involvement in regulating the formation of stamen homologous transformation into pistil traits in the wheat homologous transformation sterility-1(HTS-1).This study provides a theoretical basis for further research on the function of wheat WUS subfamily genes in wheat.
关键词
小麦/WUS基因亚家族/雄蕊/雌蕊Key words
Triticum aestivum/WUS gene subfamily/Stamen/Pistil引用本文复制引用
基金项目
国家自然科学基金(32272066)
国家自然科学基金(32060456)
四川省自然科学基金(2022NSFSC0160)
四川省科学技术厅重大科技专项子课题(2022ZDZX0014-4-4)
出版年
2024
国家科技期刊平台
NSTL
万方数据