半夏HD-Zip基因家族全基因组鉴定及表达分析
Genome Wide Identification and Expression Analysis of the HD-Zip Gene Family in Pinellia ternata
刘梦梦 1刘晓 1尤倩 1伯晨 1朱艳芳 1段永波 1薛建平 1王德信 2薛涛1
作者信息
- 1. 淮北师范大学生命科学学院/安徽省特色资源植物利用工程实验室,资源植物高效栽培与利用淮北市重点实验室,淮北 235000
- 2. 菏泽学院农业与生物工程学院,菏泽 274015
- 折叠
摘要
环境胁迫引发的半夏(Pinellia ternata)倒苗,严重限制了其产业的发展,同源异型域-亮氨酸拉链蛋白(homeodomain-leucine zipper,HD-Zip)家族基因广泛参与植物的逆境响应调控.为发掘参与半夏倒苗调控的HD-Zip基因,本研究开展了半夏基因组水平HD-Zip家族基因的鉴定分析.结果发现,半夏中共鉴定出17个HD-Zip家族基因,其中15个成员挂载至9条染色体上.进化分析显示,鉴定出的17个HD-Zip家族成员包括9个HD-Zip Ⅰ亚家族成员和8个HD-Zip Ⅱ亚家族成员,蛋自序列中均具有高度保守性的HD结构域(WFQNRR).亚细胞定位预测显示,17个HD-Zip家族成员均定位于细胞核中;共线性分析表明,8个PtHDZ基因形成4对同源基因对,且1个基因与水稻(Oryzasa tiva)之间存在共线性关系.顺式作用元件预测分析表明,鉴定的PtHDZ基因启动子均含激素、胁迫响应元件和转录因子结合位点等元件.组织表达模式分析显示,半夏HDZ家族基因在不同组织中呈现差异表达,多数基因在根和块茎中高表达;高温胁迫下表达模式分析发现,14个PtHDZs受高温诱导表达,2个PtHDZs的表达受高温抑制.本研究为解析半夏的倒苗机理及分子育种提供了基因资源.
Abstract
The sprout tumble(ST)of Pinellia ternata caused by environmental sress severely limits its industrial development,and the homeodomain-leucine zipper(HD-Zip)family genes are widely involved in the regulation of plant stress response.To explore the HD-Zip genes involved in the regulation of ST in P.ternata,the HD-Zip family genes of P.ternata were identified and analyzed at the genome level in this study.It was found that 17 HD-Zip family genes were identified in P.ternata,among them,15 of which were attached to 9 chromosomes.Evolutionary analysis showed that the identified 17 HD-Zip family members included 9 HD-Zip Ⅰ subfamily members and 8 HD-Zip Ⅱ subfamily members,all of which had highly conserved HD domains(WFQNRR)in their protein sequences.Subcellular localization prediction showed that all 17 members of the HD-Zip family were located in the nucleus,and collinearity analysis showed that 8 PtHDZ genes formed 4 pairs of homologous gene pairs,and 1 genes had a collinearity relationship with rice(Oryza sativa).The elements of hormones response,stress response,and transcription factor binding sites were contained in the promoters of PtHDZ genes via the prediction analysis of cis acting elements.Organizational expression pattern analysis revealed that the PtHDZ family genes showed differential expression in different tissues,most of which were highly expressed in roots and tubers.And the expression of 14 PtHDZs were found to be induced while 2 PtHDZs were inhibited by high temperature.This study provides genetic resources for analyzing the mechanism of ST and molecular breeding in P.ternata.
关键词
半夏/转录因子/HD-Zip家族/高温胁迫/基因表达Key words
Pinellia ternata/Transcription factors/HD-Zip family/High temperature stress/Gene expression引用本文复制引用
基金项目
国家自然科学基金(82274048)
安徽省高校自然科学研究项目(KJ2021A0532)
安徽省高校优秀创新团队(2022AH010029)
安徽省高校优秀创新团队(2023ZK016)
安徽省高校优秀创新团队(PT21110037)
出版年
2024
国家科技期刊平台
NSTL
万方数据