Technology of Tissue Culture in Caragana Korshinskii Kom.
To explore the technique systems of tissue culture and rapid propagation for Caragana Korshinskii, through the randomised block design, combine with analysis of variance, the optimal medium and culture program were studied, using the stems as explants. The experimental results indicated that the optimal method of sterilization was sterilized with alcohol 10 seconds and 0. 1 % HgC12 10 minutes in spring; The optimal medium for initial culture was MS +6 - BA 0. 2 mg-L-1 + NAA 0.1 mg·L-1 + AC 0. 3 g·L-1; The optimal medium for subculture multiplication was MS + 6 - BA 0.2 mg · L -1 + IAA 0. 2 mg · L-1 + AC 0.4 g · L -1; and the 1 /8 MS + IBA 0. 3 mg-L·1 +NAA 0. 8 mg-L·1 and sugar 15 g·L-1 was the best medium in rooting culture.
NETL
NSTL
万方数据
维普
