摘要
目的 分析云南省普洱市思茅区健康成年女性宫颈人乳头瘤病毒(HPV)感染及基因亚型分布情况,为预防女性宫颈感染HPV和疫苗研制提供科学依据.方法 对参加某项HPV疫苗临床试验的18～45岁健康女性受试者进行宫颈细胞学采样,用PCR-反向点杂交法进行HPV及基因亚型的检测.采用描述性方法对数据进行HPV感染率、基因亚型分布、单一和多重感染分布特征分析.结果 在697名18～45岁健康女性受试者中,宫颈HPV总感染率为20.80％.共检出22个HPV基因亚型,主要的高危型别为52型(15.00％)、16型(11.00％)、58型(7.00％)、53型(6.50％)和51型(6.00％);低危型别为43型(6.50％)、81型(5.50％).18～25岁年龄组HPV感染率最高(25.21％),随着年龄的增长有所下降(x2=4.490,P＞0.05),各年龄组感染型别均以52型、16型和43型为主.感染模式以单一感染(73.79％)为主,基因亚型主要为HPV52、16、58、59、56和43型;多重感染重数最多为五重感染,其中以二重感染为主(占73.68％),亚型以HPV52或16型伴其他高危型别为主;36～45岁组的多重感染率明显减少.结论 思茅区成年健康女性宫颈HPV感染率较高,感染的主要高危HPV基因亚型与全国及云南其他地区的基因亚型基本一致,但低危型以HPV43和81型为主.
Abstract
Objective To analyze the distribution of cervical human papillomavirus(HPV)infection and genotype in healthy adult women in Simao District,Yunnan Province,to provide a scientific basis for the prevention of cervical HPV infection in women and vaccine development.Methods Cervical cytology samples were taken from healthy female subjects aged 18～45 years who participated in a clinical trial of HPV vaccine,and HPV genotype were detected by PCR-reverse dot hybridization.Descriptive method was used to analyze the HPV infection rate,genotype distribution,single and multiple infection distribution characteristics.Results Among 697 healthy female subjects aged 18～45 years,the overall cervical HPV infection rate was 20.80％.A total of 22 HPV genotypes were detected,and the main high-risk genotypes were 52(15.00％),16(1 1.00％),58(7.00％),53(6.00％)and 51(6.00％).The low-risk genotypes were 43(6.50％)and 81(5.50％).The HPV infection rate in the age group of 18～25 years was the highest(25.21％),and decreased with age(x2=4.490,P＞0.05),The main types of infection in all age groups were 52,16 and 43.The infection pattern was dominated by single infection(73.79％),and the genotypes were mainly HPV 52,16,58,59,56 and 43.The most severe cases of multiple infections were five major infections,of which double infection was dominant(73.68％),and the genotypes were HPV52 or 16 with other high-risk genotypes.The rate of multiple infections decreased significantly in the 36～45 years old group.Conclusion The cervical HPV infection rate of adult healthy women in Simao District was relatively high,and the main high-risk HPV genotypes were basically the same as those in China and other areas of Yunnan,but the main low-risk types were HPV43 and 81.
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