Study on the immune mechanism of monocyte/macrophage and EBV activation in patients with drug eruption
Objective To define the expression of monocytes/macrophages and their associated receptors of different types of cutaneous drug reactions(CDR)and to clarify the effect of EBV activation on CDR.Methods Patients with CDR and healthy control group were collected and divided into maculopapular drug eruption(MPE),Stevens-Johnson syndrome(SJS)/toxic epidermal necrolysis(TEN),erythrodermic drug eruption(EDE),and control group according to their clinical manifestations.Blood counts,blood biochemistry,and expression of monocytes/macrophages and their receptors were analyzed.The peripheral blood mononuclear cells of patients with CDR and healthy control group were stimulated by EBV,and the expression of monocytes/macrophages and their receptors were analysed according to whether EBV was activated,and the differences of various detection results among all groups were compared and analyzed.Results The result showed that macrophages decreased and HLA-DR increased in CDR patients;eosinophilic granulocytes increased and M2/M1 shifted in EDE patients,ALT increased and M2/M1 slightly shifted or not shifted in MPE and SJS/TEN patients.After EBV activation,monocytes and ALT were elevated,macrophages slightly elevated,and M2/M1 shifted in CDR patients.Conclusion① Elevation of HLA-DR is one of the indicators of drug sensitization;② The pathogenesis of different CDR types is different,and M2/M1 shift can be used as an indicator of CDR types;③ Elevated monocytes and ALT should be highly vigilant against EBV activation,and M2/M1 shift is a risk indicator of EBV activation.
Cutaneous drug reactionsMonocyteMacrophageEpstein-Barr virus
万方数据
维普
