Study on the Preservation of Liquid Protease with Complex Protectant
Neutral proteases are less stable and are susceptible to physical and chemical factors such as temperature,pH and salt concentration,resulting in poor storage and transportation.In order to maintain the stability of the liquid protease preparation,a compound protectant composed of sugars,alcohols and preservatives was added to the crude enzyme solution,and the effect of the compound protectant on the stability of the liquid protease was studied.The results showed that the compound protectant with 2 g/L galactose,18%glycerol,3%sodium lactate and 1 g/L gelatin could significantly improve the enzymatic activity of protease,and the stability of liquid enzyme at different pH and temperature was improved after adding compound protectant.The stability of liquid enzyme was studied to provide reference for improving the quality of liquid enzyme products.