Based on the principle that phenylalanine and tyrosine in collagen produce quantitative endogenous fluorescence,the effect of yak hide collagen mass concentration on its self-assembly behavior and the phenomenon of fluorescence resonance energy transfer during the assembly process were studied by synchronous fluorescence spectrum and emission fluorescence spectrum,and the critical assembly mass concentration of yak hide collagen in 0.5 mol/L acetic acid was determined to be 0.77 mg/mL by calculation.The corresponding working curve between fluorescence intensity of yak hide collagen and its mass concentration of 0.10-3.00 mg/mL was established,and the efficiency of collagen passing through 0.22 μm filter membrane was quantitatively analyzed,which proved that the passing rate was linear with the natural logarithm of mass concentration.Yak hide collagen formed the complex self-assembly structure under acidic salt-free conditions,and the addition of neutral salt changed the assembly state and process.In the presence of phosphate buffer-sodium chloride,the self-assembly effect is optimum.With the increase of assembly degree,collagen gradually formed the orderly spatial structure,and the absorbance,fluorescence intensity,modulus and complex viscosity increased,while the loss coefficient decreased.
yak hidecollagenself-assemblyfluorescence analysisneutral salt
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