Determination of Peptide Antibiotics in Water by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry
Water samples were filtered using a 0.45 μm pore size membrane,followed by the addition of Na2EDTA salt to remove certain metal ions and pH adjustment to 3.Oasis HLB solid-phase extraction columns were employed for enrichment and purification,with elution using 5 mL of 0.1%formic acid in methanol and 5 mL of methanol.The samples were then concentrated using nitrogen blowdown.Ultra-high-performance liquid chromatography-tandem mass spectrometry was utilized for the detection of peptide antibiotics using a mobile phase consisting of 0.1%formic acid in acetonitrile and 0.1%formic acid in water.The results showed that within the concentration range of the standard curve,all 11 peptide antibiotics exhibited linear relationships with r2 values greater than 0.998,and the detection limits were below 1 ng/L.Low,medium,and high concentration spikes yielded average recoveries ranging from 77.7%to 98.1%,with relative standard deviations ranging from 1.5%to 14.2%.This method is characterized by its simplicity,short testing time,good accuracy and precision,and low detection limits.It allows for the simultaneous determination of 11 peptide antibiotics in water quality.
Oasis HLBwater qualitypeptide antibioticsultra high performance liquid chromatography tandem mass spectrometry
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