In order to investigate the structure and function of the Mut3 gene derived from the nitrile hydrolase mutant of Alcaligenes faecalis,the group analyzed the bioinformatics of the nitrile hydrolase specifically,including physicochemical properties,protein secondary structure,tertiary structure,conserved structural domains of the protein,transmembrane region,signal peptide,hydrophilicity and hydrophobicity on the basis of the previous studies.The bioinformatics analysis of nitrile hydrolase provides an important theoretical basis for its further molecular modification.